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Product introduction

AF488是一种胺反应性、发绿光的染料，通常用于通过伯胺 (Lys)、胺修饰的寡核苷酸和其他含胺生物分子标记蛋白质或抗体。标记在 pH 7-9 时最有效，并形成稳定的共价酰胺键。 AF 488 是一种水溶性、高光稳定性染料，具有非常高的荧光量子产率。具有绿色荧光，在 pH 4 到 pH 10 之间具有独立的 pH 值，其激发非常适合 488 nm 激光线。AF 488 染料可与多种抗体、肽、蛋白质、示踪剂和扩增底物结合，这些底物通常用于在成像和流式细胞术中产生稳定的信号。 AF 488 Ester 在结构上与 Alexa Fluor® 488 NHS Ester 相同。它的吸收/发射光谱与许多其他基于磺化罗丹明 110 核心的荧光染料的光谱完美匹配，包括 DyLight® 488, CF® 488 Dye。荧光基团标记：AF 488染料反应性基团：NHS 酯反应性：蛋白和配体、胺修饰的寡核苷酸上的伯胺偶联物的 Ex/Em：494/517 nm 消光系数：73,000 cm-1M-1

Specs ⸢ View details ⸥

Chinese name

AF 488 NHS Ester

English name

AF 488 NHS Ester

Chinese alias

AF 488 NHS 酯类

English alias

CAS number

1374019-99-4

molecular formula

C25H17N3O13S2·xC6H15N

molecular weight

631.5 (free base) g/

Exact mass

PSA

Logp

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