酸性乙醇分化液(1%)

未知

Acidic liquid alcohol differentiation(1%)

A743396-500ml

500ml

4% in PBS

室温

常规运输

室温保存，至少一年有效。

分化作用是指组织染色后用某些特定的溶液将组织过多结合的染色剂脱去，这个过程称为分化作用，所用的溶液称为分化液。在HE染色和其他染色中常用1%盐酸乙醇作为分化液，因酸能破坏苏木素的醌型结构，使组织与色素分离而退色，再进行伊红染色，才能保证细胞核与细胞浆染色的分明。酸性乙醇分化液(1%)主要由稀酸、乙醇等组成，经常用于HE染色、Masson三色染色中，更适用于要求分化力轻微的组织，是一种非常重要的辅助试剂。 注意事项： 1、密闭保存，一旦开启尽快用完，因为其有效成分易挥发。 2、为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用方法： 1、根据实验具体要求操作。 2、一般分化2～5s，立即用水或蓝化液终止。 Aladdin's Acid Alcohol Slow Differentiation Solution is generally used in hematoxylin staining, hematoxylin-eosin staining (HE staining) or Masson staining to reduce the staining background from cytoplasm, making the hematoxylin stain of nucleus more lucid.This product developed by aladdin requires about 30 seconds for sample differentiation, enabling a convenient and fine control of the differentiation. If a faster differentiation is desired, we recommend Differentiation refers to the process of using certain specific solutions to remove excess bound staining agents from tissues after staining. This process is called differentiation, and the solution used is called differentiation solution. In HE staining and other staining methods, 1% hydrochloric acid ethanol is commonly used as the differentiation medium. Acid can destroy the quinone structure of hematoxylin, causing tissue and pigment to separate and fade. Then, eosin staining is performed to ensure clear staining of the nucleus and cytoplasm. Acidic ethanol differentiation solution (1%) is mainly composed of dilute acid, ethanol, etc. It is often used in HE staining and Masson trichrome staining, and is more suitable for tissues with mild differentiation requirements. It is a very important auxiliary reagent. Matters needing attention: 1. Sealed storage, once opened, use up as soon as possible because its active ingredients are easily volatile. 2. For your safety and health, please wear lab coats and disposable gloves when operating. Usage method: 1. Operate according to the specific requirements of the experiment. 2. Generally, the differentiation lasts for 2-5 seconds and is immediately terminated with water or blue staining solution.