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Product details
名称
增强型荧光淬灭封片剂
分子类型
生物试剂/缓冲液
别名
抗荧光淬灭封片液(强)
英文名称
Enhanced Antifade Mounting Medium
货号
A752105-5ml
包装规格
5ml
级别
适用于免疫荧光(IF)
储存温度
2-8°C储存
运输条件
冰袋运输
稳定性与储存
Store at 2-8℃ long term (12 months).
产品介绍
增强型荧光淬灭封片剂(Enhanced Antifade Mounting Medium)属于抗荧光衰减封片剂(Fluorescence decay resistant Medium)的一种,是一种用于减缓荧光淬灭的封固剂,可以用于减缓各种常见荧光染料的荧光淬灭,较普通抗荧光淬灭封片剂的抗淬灭效果大大增强,一般1~2周后才会有所减弱,按每个样本需要50~100μl封片剂计算,5ml可以用于至少50样本的封片。该试剂仅用于科研领域,不适用于临床诊断或其他用途。 自备材料: 1、载玻片 2、盖玻片 3、荧光显微镜 操作步骤(仅供参考): 1、贴壁细胞样本 (1)染色完成后,吸去染色液。 (2)滴1滴 Enhanced Fluorescent Mounting Medium 于载玻片上,盖上贴有细胞的盖玻片,让细胞接触封片液,尽量避免气泡。 (3)在荧光显微镜下观察或4℃避光保存1~2周。 2、组织切片 (1)染色完成后,吸去染色液。 (2)滴1滴Enhanced Fluorescent Mounting Medium于组织切片上,盖上盖玻片,让切片接触封片液,尽量避免气泡。 (3)在荧光显微镜下观察组织切片或4℃避光保存1~2周。 3、其它样品 其它样品参考细胞样品或组织切片进行操作。 注意事项: 1、抗荧光淬灭的概念是相对的,样本染色后最好立即观察,如果没有条件,最好应在当天观察。 2、荧光物质均易发生淬灭,染色后的样品宜4℃避光保存。 3、在使用Enhanced Fluorescent Mounting Medium的情况下,虽然可减缓淬灭,仍需尽量避光。 4、用罗丹明标记的抗体不能用该封片剂封片,可以采用聚乙烯醇荧光封片剂。 5、为了您的安全和健康,请穿实验服并戴一次性手套操作。 6、试剂开封后请尽快使用,以防影响后续实验效果。
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Research chemical 5ml

Research chemical 5ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 5ml.

item number:A752105-5ml
Product model:5ml
level: 5ml
Lead Time:30days
sold 602 Items
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