白蛋白 溶液 人

A754961-50ml

50ml

10% in 0.85% sodium chloride and 0.05% sodium azide, aseptically filled

人血清白蛋白溶液可作为RPMI 1640培养基的补充剂，用于多形核细胞(PMN)的培养。也可作为培养基，用于单核细胞来源树突状细胞的分化和成熟。应用： 人血清白蛋白(HSA)是人血浆蛋白的主要成分，为单体形式的α螺旋球蛋白。多肽单链由585个氨基酸残基组成，链内含有17个二硫键桥和1个游离半胱氨酸。人血清白蛋白经过三种不同的翻译后修饰：氧化、糖基化和S-亚硝基化。修饰通常发生在球状蛋白的表面，不显著影响构象。然而，修饰强烈影响脂肪酸和药物分子的结合。 Human serum albumin (HSA) is a monomeric, globular, and α-helical protein that constitutes a major part of human blood plasma proteins. This single-chain polypeptide protein contains 585 amino acid residues and 17 internal disulfide bridges and one free cysteine. Human serum albumin undergoes three different post-translational modifications: oxidation, glycation, and S-nitrosylation. Modifications usually occur on the surface of the globular protein, and do not significantly affect conformation. However, modification strongly affects binding of fatty acids and drug molecules. Application: Albumin solution human has been used as a supplement in RPMI 1640 media during polymorphonuclear cell (PMN) incubation. It has also been used as a culture media for the differentiation and maturation of monocyte-derived dendritic cells.

人血清白蛋白(HSA)在血液的胶体渗透压调节中起重要作用。也有利于离子稳态维持，例如可进行过渡金属的运输和储存。HSA与多种离子、分子的高结合能力令其成为用于生物物理、临床和工业研究的有效分子载体和纳米溶剂。人和牛白蛋白含16％氮，通常用作蛋白质校准研究的标准品。可作为封闭剂用于蛋白质免疫印迹分析(Western blot)和酶联免疫吸附测定(ELISA)。不含球蛋白的白蛋白适用于不能存在其他蛋白的应用（如电泳）。