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Product details
名称
天青石蓝B染色液
分子类型
生物试剂/缓冲液
别名
媒介蓝14 | 天青石蓝
英文别名
Mordant Blue 14
英文名称
Celestine Blue B Staining Solution
货号
A774774-50ml
包装规格
50ml
级别
BioReagent
浓度
0.50%
储存温度
2-8°C储存,避光
运输条件
冰袋运输
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
敏感性
Light-sensitive
产品介绍
苏木素 (Hematoxylin) 和伊红 (Eosin) 联合染色简称HE染色,是病理学和组织学最常用的一种染色方法。苏木精为碱性天然染料,可使细胞核着色。细胞核内染色质的主要成分是DNA,在DNA的双螺旋结构中,两条核苷酸链上的磷酸基向外,使DNA双螺旋的外侧带负电荷,呈酸性,很容易与带正电荷的苏木精碱性染料以离子键或氢键结合而被染色。Van Gieson染色时使用的酸可脱掉大部分苏木素的颜色,以致细胞核几乎看不到,在这种情况下使用铁苏木素或天青石蓝可抵抗酸的影响而达到满意效果。 天青石蓝B染色液主要由0.5%天青石蓝B组成,多与明矾苏木素联合使用,其优势在于天青石蓝B能抗酸,且高铁盐可加强明矾苏木素与细胞核的结合力,使细胞核染色更深,因此可以达到抗酸的目的,使苏木素的颜色不易褪去。 使用说明: 1、石蜡切片脱蜡,梯度乙醇水化至水。 2、入天青石蓝B染色液,浸染5min。蒸馏水冲洗,按要求继续进行下游实验。 注意事项: 1、随着染色液放置时间的延长,染色力会有所下降,染色时间也需延长。 2、染液的使用程度越强,染色力下降越快,染色时间也就越长。 3、冰冻切片的染色时间应缩短,在无缓冲液的福尔马林溶液浸泡时间较长的组织及脱钙组织染色时间应延长。
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Research chemical 50ml; 0. 50%

Research chemical 50ml; 0. 50%; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 50ml; 0. 50%.

item number:A774774-50ml
Product model:50ml
level: 50ml; 0. 50%
Lead Time:30days
sold 817 Items
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