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Product details

名称

Recombinant alpha Tubulin Antibody

分子类型

抗体

别名

英文别名

物理外观

Liquid

货号

Ab088465-10μl

包装规格

10μl

级别

重组

浓度

见COA

储存温度

-20°C储存,避免反复冻融

运输条件

超低温冰袋运输

稳定性与储存

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

储存缓冲液

10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4.

防腐剂

0.02% Sodium azide

生化机理

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Post-translational: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

免疫原

A synthetic peptide derived from human alpha 4A Tubulin (AA 10-51).

宿主种属

兔(Rabbit)

种属反应性

人(Human), 大鼠(Rat), 小鼠(Mouse) | 人(Human),大鼠(Rat),小鼠(Mouse)

特异性

alpha Tubulin

阳性对照

WB: Mouse brain, HeLa, Hep G2, Jurkat, HEK293, NIH/3T3 and C6 lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa, Hep G2 cells.

SDS-PAGE

150 kDa

偶联

Unconjugated

亚型

Rabbit IgG

克隆类型

重组抗体

轻链亚型

kappa

纯化方法

Protein A purified

Format

Whole IgG

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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