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Product details

名称

CDC25A Antibody

分子类型

抗体

别名

细胞分裂周期蛋白25抗体

英文别名

物理外观

Liquid

货号

Ab095599-10μl

包装规格

10μl

浓度

见COA

储存温度

-20°C储存,避免反复冻融

运输条件

超低温冰袋运输

稳定性与储存

短期储存2-8℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。

储存缓冲液

10mM PBS, 40% Glycerol, 0.05% Sodium azide, pH 7.4

防腐剂

0.05% Sodium azide

生化机理

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. Post-translational: Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.

免疫原

Fusion protein of human CDC25A.

宿主种属

兔(Rabbit)

种属反应性

人(Human), 大鼠(Rat), 小鼠(Mouse) | 人(Human),大鼠(Rat),小鼠(Mouse)

特异性

CDC25A

阳性对照

WB: Mouse heart lysates. IHC: Human cervical cancer tissue.

SDS-PAGE

150 kDa

偶联

Unconjugated

亚型

Rabbit IgG

克隆类型

多克隆抗体

纯化方法

Antigen affinity purified

Format

Whole IgG

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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