S100A9 Mouse mAb

抗体

S100A9抗体 | S100A9单克隆抗体 | S100钙结合蛋白A9抗体

Leukocyte L1 complex heavy chain antibody | 60B8AG antibody | CAGB antibody | Calgranulin B antibody | Calgranulin-B antibody | Calprotectin L1H subunit antibody | CFAG antibody | CGLB antibody | Cystic fibrosis antigen B antibody | L1AG antibody | Leukoc

Liquid

Ab126262-10μl

10μl

已验证

见COA

-20°C储存,避免反复冻融

超低温冰袋运输

短期储存4℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。

10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3.

0.02% Sodium azide

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Participates also in regulatory T-cell differentiation together with CD69. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Has transnitrosylase activity; in oxidatively-modified low-densitity lipoprotein (LDL(ox))-induced S-nitrosylation of GAPDH on 'Cys-247' proposed to transfer the NO moiety from NOS2/iNOS to GAPDH via its own S-nitrosylated Cys-3. The iNOS-S100A8/A9 transnitrosylase complex is proposed to also direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif.

Recombinant full length protein of human S100A9 produced in SF9 cell.

小鼠(Mouse)

人(Human)

S100A9

WB: HEK293T transfected with pCMV6-ENTRY S100A9 cDNA for 48 hrs; MCF7 lysates. IHC: Human prostate, prostate carcinoma, lymphoma, tonsil tissues.

150 kDa

Unconjugated

Mouse IgG2a | Rabbit IgG

单克隆抗体

Protein A purified

OTI13E12

Whole IgG