Ankaflavin, isolated from Monascus-Fermented red rice, is an orally active PPARγ agonist. Ankaflavin exhibits selective cytotoxic effect and induces cell death through apoptosis on cancer cells. Ankaflavin has anti-inflammatory, anti-cancer, antiatherosclerotic, and hypolipidemic effects In Vitro Ankaflavin (0-50 µg/mL, 48 h) shows cytotoxicity against cancer cells with no significant toxicity toward normal cells. Ankaflavin (0-30 µg/mL, 0-48 h) arrests Hep G2 cell cycle at sub-G1 phase in a dose- and time-dependent manner. Ankaflavin (25 µg/mL, 48 h) induces Hep G2 cell apoptosis. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cytotoxicity AssayCell Line: A549, Hep G2, MRC-5 and WI-38 Concentration: 1, 10, 25, and 50 µg/mL Incubation Time: 48 h Result: Showed cytotoxicity against A549 and Hep G2 cells in a dose-dependent manner with no significant toxicity toward normal cells (MRC-5 and WI-38). Cell Cycle AnalysisCell Line: Hep G2 cells Concentration: 15, 20, 25, and 30 µg/mL Incubation Time: 12, 24, 36, and 48 h Result: Induced a distinct sub-G1 peak in Hep G2 cells in a dose- and time-dependent manner. Apoptosis AnalysisCell Line: Hep G2 cells Concentration: 25 µg/mL Incubation Time: 48 h Result: Exhibited significant chromatin condensation (fluorescent spot) through Hoechst staining. In Vivo Ankaflavin (10 mg/kg; p.o.; daily for 28 days) shows antidiabetic and anti-inflammatory activity, improves liver function and pancreatic function. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Wistar rats (4 weeks of age), diabetes was induced by treating them with Methylglyoxal (MG) (600 mg/kg; oral) for 4 weeksDosage: 10 mg/kg Administration: Oral administration for 28 days Result: Exerted PPARγ agonist activity. Effectively reduced AGE (advanced glycation end-products) levels in serum, liver, and pancreas of MG-induced rats. Form:Solid IC50& Target:PPARγ