BODIPY 581/591 C11 is a BODIPY borofluoroprene derivative with good light stability and low fluorescence artifacts. BODIPY 581/591 C11 can be used for study lipid peroxidation and antioxidant properties in living cells, or detect ferroptosis by reaction with hydroxyl radicals. BODIPY 581/591 C11 is emitted at 591 nm (reduced prototype), or redshifted to 510 nm (oxidized type). The excitation wavelengths were 581 nm (reduced prototype) and 500 nm (oxidized type). In Vitro Preparation of the stock solution Dissolve 1mg BODIPY 581/591 C11 in 0.1983 mL DMSO to obtain 10 mM of BODIPY 581/591 C11. Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of BODIPY 581/591 C11 working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 2-10 μM of BODIPY 581/591 C11 working solution. Note: Please adjust the concentration of BODIPY 581/591 C11 working solution according to the actual situation. Cell staining 2.1 Cell preparation: For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of BODIPY 581/591 C11 working solution, and then incubate at room temperature for 30 minutes. 2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. Precautions 1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles. 2. Please adjust the concentration of BODIPY 581/591 C11 working solution according to the actual situation. 3. This product is for R&D use only, not for drug, household, or other uses. 4. For your safety and health, please wear a lab coat and disposable gloves to operate. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid