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Product details

名称

BCA蛋白定量试剂盒

分子类型

试剂盒

英文名称

BCA Protein Assay Kit

货号

B665595-500T

包装规格

500T

储存温度

2-8°C储存

运输条件

冰袋运输

产品介绍

BCA（Bicinchoninic acid）法是⽬前应⽤⽐较⼴泛的蛋⽩质浓度测定⽅法。基于双缩脲反应，即在碱性环境下蛋⽩质将 Cu2+还原成 Cu+，产⽣⼀种紫蓝⾊复合物，在 562nm 处有⾼的吸光值，该反应产物的量与蛋⽩质浓度成正⽐。BCA 蛋⽩浓度测定法实现了蛋⽩质浓度测定的简便、灵敏、快速和稳定性。试剂盒中提供的蛋⽩标准品为⽤户制作标准曲线提供了便利。该 BCA 蛋⽩浓度测定试剂盒可⽤于⽐⾊⽫法检测，也可⽤于微孔板法检测。前者虽需较⼤量（100μL）的蛋⽩样品，但由于其在检测中使⽤蛋⽩样品与 BCA ⼯作液的⽐率为 1:20（v/v），从⽽降低⼲扰物质带来的影响。后者操作简单⽅便，仅需少量（10-25μL）的蛋⽩样品。不过，由于其在检测中使⽤蛋⽩样品与 BCA ⼯作液的⽐率为 1:8（v/v），某种程度上限制⼲扰物质的承受浓度以及降低最低检测⽔平。组分组分

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