BCECF细胞渗透性酯混合物

B755399-1mg

1mg

A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.

BCECF的细胞可渗透酯衍生物的可变混合物，其被胞质酯酶水解以产生细胞内捕获的指示剂BCECF。已用于监测哺乳动物成纤维细胞，胃细胞，淋巴细胞，肌细胞和远曲小管中细胞内pH值的变化。BCECF（目录编号216252）的细胞可渗透酯衍生物的可变混合物，其被胞质酯酶水解以产生细胞内捕获的指示剂BCECF。已用于监测哺乳动物成纤维细胞，胃细胞，淋巴细胞，肌细胞和远曲小管中细胞内pH值的变化。典型的上样缓冲液在不含氨基酸的无血清培养基中包含1-10 µM BCECF/AM，并含有伯胺或仲胺的缓冲液。 A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines. A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.

主要靶标 监测细胞内pH值的变化产物不与ATP竞争。可逆：否细胞可渗透性：是