脑心浸液琼脂

Brain Heart Infusion Agar

B755820-500g

500g

适用于微生物学

For the cultivation of fastidious, pathogenic bacteria, yeasts, and molds

室温

常规运输

BHI 琼脂是一种通用培养基，用于从临床标本中初步分离致病性需氧菌，以及培养和维持食品、动物饲料、水和其他样品中的难养微生物。添加到基础培养基中的脱纤维羊血为难养真菌微生物提供了必需的生长因子。应用： Rosenow 通过将脑组织添加到葡萄糖琼脂中设计了原始培养基。这些培养基营养丰富步步高并且缓冲良好，可支持多种微生物的生长，例如链球菌、肺炎球菌、脑膜炎球菌等。加入腹水可以培养淋球菌。由于添加了10%脱纤维羊血，它可用于分离培养荚膜组织胞浆菌和其它病原真菌。该培养基用于选择性分离难养真菌（尤其是荚膜组织胞浆菌和芽生菌），通过添加以下推荐的成分混合物，几乎可以完全抑制伴生细菌和腐生酵母菌和霉菌的生长：每升 12 毫克青霉素和 40 微克链霉素； 每升 50 毫克氯霉素和 500 毫克放线菌酮； 每升 50 毫克庆大霉素和/或 50 毫克氯霉素。当添加血液时，由于其葡萄糖含量，该培养基不太适合鉴定溶血形式。在处理荚膜组织胞浆菌时应格外小心，以避免其感染性孢子的传播。培养物应在封闭的过滤空气柜中检查。 Rosenow devised the original medium by adding brain tissue to dextrose agar. These media are nutritious and well buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci , etc. Addition of ascites permits the cultivation of Gonococci . With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other pathogenic fungi. This medium is to be used for the selective isolation of fastidious fungi (especially of Histoplasma capsulatum and Blastomyces ), the growth of the accompanying bacterial and saprophytic yeasts and molds can be almost completely suppressed by adding the following recommended components mixtures: 12 mg Penicillin and 40 ug Streptomycin per liter; 50 mg Chloramphenicol and 500mg Cycloheximide per liter; 50 mg Gentamicin and/or 50 mg Chloramphenicol per liter. This medium is less suited for identifying haemolytic forms when blood is added due to its glucose content. While handling Histoplasma capsulatum extreme care should be taken to avoid dissemination of its infective spores. The culture should be examined in a closed filtered air cabinet. Application: BHI agar is a general-purpose medium used for primary isolation of pathogenic aerobic bacteria from clinical specimens and for the cultivation and maintenance of fastidious microorganisms from food, animal feed, water, and other samples Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms.

7.2-7.6 (25 °C)