梭菌蛋白酶 来源于溶组织梭菌

Endoproteinase-Arg-C;Clostridiopeptidase B;Proteinase from Clostridium histolyticum

Endoproteinase-Arg-C;Clostridiopeptidase B;Proteinase from Clostridium histolyticum

C128677-10mg

10mg

EnzymoPure™

≥50 units/mg dry weight

Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.A protease that cleaves proteins on the carboxyl bond of arginine Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory. A protease that cleaves proteins on the carboxyl bond of arginine

来自组织溶解梭菌的 Clostripain 由两条多肽链组成，分子质量分别为 41.7 kDa 和 15.4 kDa。Clostripain 对 Arg-Xaa 肽键具有高度的底物特异性。因此，梭菌毒素被认为是一种潜在的蛋白质测序酶。人们还将其作为一种催化剂进行研究，以缩合含有 Arg-Pro 键的重要药物肽。