胶原酶 AF-1 来源于溶组织梭菌

Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene

C278962-2000U

2000U

EnzymoPure™

≥ 3.00 U/mg

描述 胶原酶AF-1使用完全不含任何动物成分的生产工艺衍生自溶组织梭状芽胞杆菌。因此，排除了潜在的动物源性病原体传播的任何风险。这种高度纯化的胶原酶，通常与中性蛋白酶AF结合，适用于从几个组织中分离敏感细胞。已经证明，不含动物的酶对于从拟移植的人胰腺中分离朗格汉斯胰岛以及从脂肪组织和肿瘤细胞中分离基质血管级分（SVF）都是非常有效的。 应用 胶原酶AF-1可用于多种细胞分离。我们更大的包装规格为2000 U，是人类胰岛分离的理想选择，而较小的包装规格为150 U，可用于肿瘤细胞分离，从脂肪组织中分离TIL或SVF。 规格 活性≥3.00 U / mg *。 内毒素≤10.0 EU ** / mg（Ph.Eur。） 微生物学检查：总需氧微生物数（TAMC），总酵母/霉菌总数（TYMC），无梭状芽胞杆菌。 最终产品经过0.22 µm过滤。 （Clostridiopeptidase A，胶原酶A） EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1] 参考和定义 * 单位定义：胶原酶：1 U在25°C，pH 7.1下每分钟催化1 µmol 4-苯基偶氮苄氧基羰基-L-脯氨酰-L-亮氨酰甘氨酰-L-脯氨酰-D-精氨酸的水解。 **内毒素： 欧洲药典 [1内毒素单位等于WHO批准的参考标准内毒素（RSE）的1国际单位]。 References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51 Collagenase AF-1 is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF is suitable for the dissociation of sensitive cells from several tissues. The animal-free enzymes have been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells. Application Collagenase AF-1 can be used for several cell isolations. Our bigger packaging size of 2000 U is ideal for human islet isolation, whereas the smaller size of 150 U can be used for tumor cell isolation, isolation of TILs or SVF from adipose tissue. Specifications Activity (Wünsch) ≥ 3.00 U/mg*. Endotoxin ≤ 10.0 EU**/mg (Ph. Eur.) Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia. Endproduct is 0.22 µm filtered. (Clostridiopeptidase A, Collagenase A) EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1] References and Definitions *Unit definitions:Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. **Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)]. References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51