胶原酶 NB 6 来源于溶组织梭菌

Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene

C278976-100mg

100mg

GMP

≥ 0.100 U/mg

描述 胶原酶NB 6是一种粗制胶原酶，包含胶原蛋白水解和其他酶促活性，包括梭菌蛋白酶和中性蛋白酶。 这些活动的均衡比例确保了温和有效的组织解离。 规格 包含I类和II类胶原酶（1）和均衡比例的蛋白水解副活性。 活性：≥0.100 U / mg 微生物学检查：无菌，无梭菌，异常毒性（一般安全性测试；欧洲药典）。 根据Ph.Eur。 （Clostridiopeptidase A） EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1] 应用领域 胶原酶NB 6适用于从各种临床用途的组织中分离细胞。 参考和定义 单位定义：1 U催化 在25°C，pH 7.1下，每分钟1 µmol 4-苯基偶氮苄氧基羰基-L-脯氨酰基-L-亮氨酰甘氨酰-L-脯氨酰基-D-精氨酸。 References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51 Collagenase NB 6 is a crude collagenase that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. Specifications Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities. Activity (Wünsch): ≥ 0.100 U/mg Microbiological examination: Sterility, absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.). Sterile according to Ph. Eur. (Clostridiopeptidase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1] Applications Collagenase NB 6 is suitable for cell isolation from various tissue types intended for clinical applications. References and Definitions Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51