胶原酶 NB 1 来源于溶组织梭菌

Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene

C278986-2000U

2000U

EnzymoPure™

≥ 3.00 U/mg

描述 胶原酶NB 1在色谱上得到高度纯化；因此，它具有很高的胶原蛋白分解活性。它基本上没有其他酶促活性，如梭菌蛋白酶，类胰蛋白酶活性和中性蛋白酶，以及内毒素。 规格 包含经色谱纯化的I类和II类胶原酶（1）。 基本上不含clostripain，胰蛋白酶样蛋白酶和中性蛋白酶。 小瓶中含有不少于2000 PZ U胶原酶。 活性（Wünsch）：≥3.00 U / mg 内毒素：≤10.0 EU / mg（Ph。Eur。） （Clostridiopeptidase A） (Clostridiopeptidase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1] 应用 胶原酶NB 1主要与中性蛋白酶NB结合使用，适用于从几种组织类型中分离细胞。 参考和定义 单位定义： 胶原酶： 1 U在25°C（pH 7.1）下每分钟催化1 µmol 4-苯基偶氮苄氧基羰基-L-脯氨酰基-L-亮氨酰甘氨酰-L-脯氨酰基-D-精氨酸的水解。 内毒素： 欧洲药典 （1个内毒素单位等于WHO批准的参考标准内毒素（RSE）的1个国际单位）。 References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51. Collagenase NB 1 is chromatographically highly purified; therefore it contains a very high collagenolytic activity. It is largely free from additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as endotoxins. Specifications Contains chromatographically highly purified class I and class II collagenase (1). Largely free from clostripain, trypsin-like protease and neutral protease. Vial contains not less than 2000 PZ U collagenase. Activity (Wünsch): ≥ 3.00 U/mg Endotoxin: ≤ 10.0 EU/mg (Ph. Eur.) (Clostridiopeptidase A) EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1] Application Collagenase NB 1 is, mostly in combination with Neutral Protease NB, suitable for cell isolation from several tissue types. References and Definitions Unit definition: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.