Cas9 Nuclease (SpCas9)

Cas9 核酸酶 (SpCas9)

CRISPR-associated endonuclease Cas9 | Csn1

C744422-50pmol

50pmol

无动物源

1μM (158ng/μL )

本公司生产的 Cas9 Nuclease (SpCas9)，即 CRISPR-associated endonuclease Cas9 也称 Csn1，是本公司自主研发的技术平台表达、纯化获得 的一种来源于 Streptococcus pyogenes，能在 gRNA 引导下序列特异性地切割双链 DNA 的核酸内切酶。本产品可以用于体外筛选高效的 guide RNA (gRNA)序列、特定 DNA 序列在 gRNA 引导下的剪切、含有特定序列的双链环形 DNA 的线性化等用途。CRISPR/Cas9 是一项突破性的基因组 编辑技术，操作便捷，应用广泛。CRISPR (clustered regularly interspaced short palindromic repeats)是一种原核生物利用 RNA 引导的 DNA 核酸酶 Cas9 对外源的噬菌体或病毒核酸进行基因沉默的获得性免疫系统(adaptive immune system)，后续在此基础上逐渐发展为广泛应用于原 核和真核生物的越来越成熟的基因编辑技术。该技术能够在 gRNA 引导下通过 Cas9 对原核和真核生物的基因组 DNA 的靶向序列进行位点特异性 的切割，然后通过易错修复(error-prone repair)或同源重组(homologous recombination)在切割位点改变或插入序列来产生移码突变，从而通过 基因编辑而实现基因敲除。其中 gRNA 确保识别位点的特异性。随着 CRISPR 技术的发展，该技术目前不仅可以实现基因敲除，还可以实现基因的 点突变、插入突变等多种突变方式，特别是在临床应用方面可以用于修复不良突变等。同时通过构建没有内切酶活性的 Cas9 突变体 dCas9，通过 与 dCas9 直接融合表达或间接招募转录激活或转录抑制因子，可以实现 sgRNA 靶向基因的转录激活或转录抑制。 纯度（Purity）： 不含 DNA 外切酶，不含非 gRNA 依赖的 DNA 内切酶，不含 RNA 酶。 组分和说明 C744422

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids)CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity .Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference).

重组表达

E. coli Accession #: Q99ZW2.1 | E. coli

化脓性链球菌血清型M1(Streptococcus pyogenes serotype M1)

Yes

Yes