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Product details

名称

Cre重组酶

别名

Cre重组酶

英文别名

‌Cyclization Recombination Enzyme | Type I topoisomerase | tyrosine recombinase

货号

C745028-50U

包装规格

50U

级别

无动物源

浓度

1.0 U/μL

产品介绍

本公司生产的Cre重组酶是自主研发的，通过技术平台表达、纯化获得的高品质Cre重组酶。Cre重组酶常用于两个同方向的loxP位点之间的基因序列删除。Cre重组酶是来源于大肠杆菌噬菌体P1的一种I型拓朴异构酶(TypeItopoisomerase)，也是一种酪氨酸重组酶(tyrosinerecombinase)，能识别34bp的loxP位点(两端为两个13bp的反向重复序列(invertedrepeats)，并能催化loxP位点之间的DNA发生重组；重组产物根据loxP位点的位置和相对方向的不同而不同，两个含单loxP位点的DNA将发生融合：两个同方向的loxP位点间的DNA将以环状形式被切割，而两个反向loxP位点间的DNA序列将被翻转。失活或抑制(Inactivation or inhibition)：70℃加热10分钟失活。组分和说明 C745028

生物活性

One unit is defined as the amount of enzyme required to achieve the maximum site-specific recombination of 250ng of control DNA in 30 minutes at 37ºC in a 50μl reaction system. The maximum recombination can be analyzed by agarose gel electrophoresis or by screening on the corresponding resistance plates after transformation of the reactions.

来源

重组表达

表达系统

E. coli Accession #: Q5QBM7 | E. coli

种属

大肠杆菌噬菌体P1

无动物源

Yes

无载体

Yes

Safety

「No Dangerous Attributes」

Related

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

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「No related articles yet」

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Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number：C745028-50U

Product model：50U

level： No animal source

Lead Time：30days

sold 637 Items

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Specifications：

50U

Qty

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