L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity. In Vitro L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI. L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding. L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation. L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HeLa cells Concentration: 0-30 μM Incubation Time: 5 days Result: Reduced cell number by about 70% at 20 μM. Cell Proliferation AssayCell Line: CH12F3 WT and CH12F3Δ/Δ cells Concentration: 0-100 μM Incubation Time: 72 h Result: Had great effect on the proliferation and survival of the parental CH12F3 cells. Form:Solid