Collagenase - Elastase blend

Collagenase - Elastase blend

C754897-1vial

1vial

用于细胞培养

Tissue dissociation blend, lyophilized powder

-20°C储存

超低温冰袋运输

The Collagenase-Elastase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Elastase: a serine protease that also hydrolyzes amides and esters. It is produced in the pancreas as an inactive zymogen and activated in the duodenum by trypsin. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Elastin, a matrix component of tissues involved in stretching processes, is found in highest concentrations in the elastic fibers of connective tissues. Elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin. Elastase is the enzyme of choice for the isolation of Type II cells from the lung. The Collagenase-Elastase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Elastase: a serine protease that also hydrolyzes amides and esters. It is produced in the pancreas as an inactive zymogen and activated in the duodenum by trypsin. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Elastin, a matrix component of tissues involved in stretching processes, is found in highest concentrations in the elastic fibers of connective tissues. Elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin. Elastase is the enzyme of choice for the isolation of Type II cells from the lung.

胶原酶从组织中有效释放细胞需要胶原酶的作用。胶原蛋白酶由每摩尔酶中的四克原子钙（Ca2 + ）激活。最佳 pH 值为 6.3-8.8。这种酶通常用于消化组织样本中的结缔成分，以释放单个细胞。胶原酶处理会导致一些细胞死亡。消化时通常使用 0.1 至 5 毫克/毫升不等的浓度。反应时间从 15 分钟到数小时不等，可获得满意的细胞解离效果，但不会造成过多细胞死亡。弹性蛋白酶：水解弹性纤维的特殊蛋白质弹性蛋白，并消化血红蛋白、酪蛋白和纤维蛋白。 它能在疏水性小氨基酸（如 Ile、Gly、Ala、Ser、Val 和 Leu）的羧基侧裂解蛋白质。该酶还能水解酰胺和酯类，如 N-苯甲酰基-L-丙氨酸甲酯。最佳 pH 值为 8.0-8.5。它不需要任何激活剂，但会受到氟磷酸二异丙酯、苯甲基磺酰氟、α2-巨球蛋白、α1-抗胰蛋白酶、磺酰氟、对二硝基苯二乙基磷酸酯和高浓度盐的抑制。 它能在疏水性小氨基酸（如 Ile、Gly、Ala、Ser、Val 和 Leu）的羧基侧裂解蛋白质。该酶还能水解酰胺和酯类，如 N-苯甲酰基-L-丙氨酸甲酯。