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名称

胶原酶来源于溶组织梭菌

货号

C754901-5g

包装规格

浓度

Blend Type L, ≤1.0 FALGPA units/mg solid

产品介绍

来自溶组织梭菌的胶原酶或梭菌肽酶A已被用于研究基于苯二胺芥的大分子抗肿瘤衍生物的合成和体外评价。梭菌肽酶A也已用于研究胶原酶对烧伤伤口的酶促清创术作用。 Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess the synthesis and in vitro evaluation of macromolecular antitumour derivatives based on phenylenediamine mustard. Clostridiopeptidase A has also been used in a study to investigate the enzymatic debridement of burn wounds with collagenase.

生化机理

从组织中进行细胞的有效释放需要胶原酶和中性蛋白酶的作用。胶原酶被每克摩尔酶 4 克原子钙（Ca 2+ ）活化。培养滤液被认为含有至少7种不同蛋白酶，分子量范围为68-130 kDa。最适pH为6.3-6.8。该酶通常用于消化组织样品中的结缔组分以释放单个细胞。已知乙二醇-双（β-氨基乙基醚）-N，N，N′，N′-四乙酸（EGTA）4；β-巯基乙醇；还原型谷胱甘肽；巯基乙酸，钠；和2，2′-联吡啶基；8-羟基喹啉抑制酶活性。每摩尔胶原蛋白酶被4g原子钙活化。它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。

Safety

「No Dangerous Attributes」

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