胶原酶 来源于溶组织梭菌

C754905-7.5KU

7.5KU

purified by chromatography,≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder

来自溶组织梭状芽孢杆菌的胶原酶可用于从许多类型的动物组织中分离细胞。它可用于分解人肿瘤、小鼠肾脏、人成年/胎儿脑、肺和许多其他组织。它也可以用于肝脏和肾脏的灌注研究、胰腺的消化，非实质大鼠肝细胞的分离以及肝细胞的制备。来自溶组织梭状芽孢杆菌的胶原酶可用于：从胶原蛋白凝胶中回收中性粒细胞将乳腺肿瘤活检组织分散成单细胞从子宫内膜活检组织中分离人原代子宫内膜基质细胞（HESC）应用： 来自溶组织梭状芽胞杆菌的胶原酶由胶原酶 G 和 H 组成。胶原酶具有 N 端活化剂结构域和 C 端肽酶结构域。胶原结合域（CBD）和多囊肾疾病样（PKD 样）域位于肽酶域中。胶原酶模块在与钙结合时发生结构变化。 Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding. Application: Collagenase from Clostridium histolyticum has been used: for the recovery of neutrophils from collagen gels for the dissociation of breast tumor biopsies into single cells for the isolation of primary human endometrial stromal cells(HESC) from endometrial biopsies Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, human adult/fetal brain, lung and many other tissues. It may also be used in liver and kidney perfusion studies, for the digestion of pancreas, isolation of nonparenchymal rat liver cells and for hepatocyte preparation.

粗胶原酶是溶组织梭状芽胞杆菌分泌的酶的混合物（主要是蛋白酶）。有效地从组织中释放细胞需要胶原酶和中性蛋白酶的共同作用。每摩尔酶用 4 克原子钙 (Ca 2+ ) 激活胶原酶。一般认为培养滤液至少含有 7 种不同的蛋白酶，分子量范围为 68-130 kDa。每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。