Collagenase-DNase I blend

Collagenase-DNase I blend

C754909-1vial

1vial

用于细胞培养

Tissue dissociation blend, lyophilized powder

-20°C储存

超低温冰袋运输

The collagenase-DNase I blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Deoxyribonuclease I (DNase I). DNase I, is an endonuclease of the DNase family, with a specific role for the degradation of DNA. DNase I is one of the most well characterized endonucleases of mammalian origin. During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells, so addition of DNase I to our blend leads to a degradation of this extracellular DNA, thereby avoiding the loss of cells from undesired clumping. The collagenase-DNase I blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Deoxyribonuclease I (DNase I). DNase I, is an endonuclease of the DNase family, with a specific role for the degradation of DNA. DNase I is one of the most well characterized endonucleases of mammalian origin. During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells, so addition of DNase I to our blend leads to a degradation of this extracellular DNA, thereby avoiding the loss of cells from undesired clumping.

胶原酶 从组织中有效释放细胞需要胶原酶的作用。胶原蛋白酶由每摩尔酶中的四克原子钙（Ca2+）激活。最佳 pH 值为 6.3-8.8。这种酶通常用于消化组织样本中的结缔成分，以释放单个细胞。胶原酶处理会导致一些细胞死亡。消化时通常使用 0.1 至 5 毫克/毫升不等的浓度。反应时间从 15 分钟到数小时不等，可获得满意的细胞解离效果，但不会造成过多细胞死亡。DNaseDNase I 是一种内切酶，可作用于与嘧啶相邻的磷酸二酯键，生成具有末端 5′-磷酸的多核苷酸。在 Mg2+ 存在的情况下，DNA 酶 I 可独立地裂解 DNA 的每一条链，裂解位点是随机的。在 Mn2+ 存在的情况下，两条 DNA 链会在大致相同的位置被裂解。最佳 pH 值为 7 至 8。Mn2+、Ca2+、Co2+ 和 Zn2+ 等二价阳离子是该酶的活化剂。浓度为 5 mM 的 Ca2+ 能稳定该酶，使其不被蛋白酶消化。牛胰腺中的 DNAse I 由 A、B、C 和 D 四种色谱法可区分的成分组成，它们的摩尔比为 4:1:1。其中只有少量的 D。已知 2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS）和肌动蛋白会抑制酶的活性。