Collagenase - Hyaluronidase blend

Collagenase - Hyaluronidase blend

C754911-1vial

1vial

用于细胞培养

Tissue dissociation blend, lyophilized powder

-20°C储存

超低温冰袋运输

The Collagenase-Hyaluronidase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Hyaluronidase. Hyaluronidase is a hyaluronic acid-metabolizing enzyme, who degrades Hyaluronic Acid. Hyaluronic Acid increase tisue and membranes permeability and is one of the main components of the extracellular matrix, contribuing significantly to cell proliferation and migration. Combination of Collagenase and Hyaluronidase is usually used for the isolation of cells from the extracellular matrix. The Collagenase-Hyaluronidase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Hyaluronidase. Hyaluronidase is a hyaluronic acid-metabolizing enzyme, who degrades Hyaluronic Acid. Hyaluronic Acid increase tisue and membranes permeability and is one of the main components of the extracellular matrix, contribuing significantly to cell proliferation and migration. Combination of Collagenase and Hyaluronidase is usually used for the isolation of cells from the extracellular matrix.

胶原酶从组织中有效释放细胞需要胶原酶的作用。胶原蛋白酶由每摩尔酶中的四克原子钙（Ca2+）激活。最佳 pH 值为 6.3-8.8。这种酶通常用于消化组织样本中的结缔成分，以释放单个细胞。胶原酶处理会导致一些细胞死亡。消化时通常使用 0.1 至 5 毫克/毫升不等的浓度。反应时间从 15 分钟到数小时不等，可获得满意的细胞解离效果，但不会造成过多细胞死亡。透明质酸酶：降解透明质酸并随机裂解透明质酸、软骨素和软骨素硫酸盐中的β-N-乙酰己糖胺-(1-4)-糖苷键。