胶原酶 来源于溶组织梭菌

C754917-50mg

50mg

无菌过滤

suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid,≥125 CDU/mg solid

胶原酶已被用于制备动脉组织，用于研究晚期糖基化终产物（AGE）。该酶还与其他蛋白酶一起用于人肿瘤、小鼠肾、人脑、肺上皮和许多其他组织的解聚。它在肝脏和肾脏灌注研究，胰腺消化和非实质肝细胞的分离中也是有效的。当测试该酶是否适合释放肝细胞时。对于每只大鼠肝脏，胶原酶以约 1mg/mL 使用，总体积为 100mL。胶原酶（源于溶组织梭菌）用于解离组织样本，如脾脏和睾丸组织。应用： 溶组织梭菌含有两种胶原酶，可剪切胶原酶三螺旋不同的切割位点。切割效率比哺乳动物胶原酶更高。梭菌胶原酶与气性坏疽的细菌侵袭有关。 Clostridium histolyticum consists of two classes of collagenases, which cleaves at multiple cleavage sites within the collagen triple helix. It is more effective than mammalian collagenases. Clostridial collagenase is implicated in bacterial invasion in gas gangrene. Application: Collagenase from Clostridium histolyticum has been used to dissociate tissue samples such as spleens and testicular tissue . Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. When this enzyme is tested for suitability for the release of hepatocytes. The collagenase is used at approximately 1 mg/mL in a total volume of 100 mL for each rat liver.

从组织中有效释放细胞需要胶原酶和中性蛋白酶的作用。胶原酶被每克摩尔酶 4 克原子钙（Ca 2+ ）活化。培养滤液被认为含有至少 7 种不同的蛋白酶，分子量范围为 68-130 kDa（最适 pH 值：6.3-8.8）。该酶通常用于消化组织样品中的结缔组分以释放单个细胞。胶原酶治疗可导致一些细胞死亡。通常，用于消化的浓度为 0.1 至 5mg/mL。反应持续时间从 15 分钟到几小时不等，这使得能够有效地解离细胞而不会导致过多的细胞死亡。具有钙和 BSA 的 Krebs Ringer 缓冲液是优选的，并且 Zn 2+ 是其活性所必需的。每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。