胶原酶 来源于溶组织梭菌

梭菌肽酶 A

C754933-25mg

25mg

天然

suitable for release of physiologically active rat hepatocytes, 0.5-5.0 FALGPA U/mg solid, ≥125 CDU/mg solid

来自组织溶梭菌的胶原酶已被用于：制备用于消化肿瘤组织的胶原酶溶液作为磷酸盐缓冲生理盐水的补充剂用于消化已切除的肠系膜脂肪组织作为罗斯韦尔公园纪念研究所1640培养基（RPMI-1640）的补充剂用于消化结肠段用于消化子宫内膜组织制备用于消化去被膜的睾丸的酶A和酶B。胶原酶已被用于制备动脉组织，用于研究晚期糖基化终产物（AGE）。该酶还与其他蛋白酶一起用于人肿瘤、小鼠肾、人脑、肺上皮和许多其他组织的解聚。它在肝脏和肾脏灌注研究，胰腺消化和非实质肝细胞的分离中也是有效的。该酶经过了肝细胞释放的适用性测试，针对每只大鼠肝脏的100 mL总体积中的浓度大约为1 mg/mL。适用于制备用于测序的单细胞悬液。 Collagenase from Clostridium histolyticum has been used: in the preparation of collagenase solution for the digestion of tumor tissues as a supplement in phosphate-buffered saline for the digestion of extirpated mesenteric fat tissue as a supplement in Roswell Park Memorial Institute-1640 medium (RPMI-1640) for the digestion of colon segments for the digestion of endometrium tissues in the preparation of enzyme A and enzyme B for the digestion of decapsulated testes Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. This enzyme is tested for suitability for the release of hepatocytes, at approx. 1 mg/mL conc., in a total volume of 100 mL for each rat liver. Suitable for use in preparation of single cell suspension for sequencing.

组织溶梭菌产生两类胶原酶。梭菌胶原酶比哺乳动物胶原酶更高效。它在胶原三螺旋中的多个裂解位点进行裂解。梭菌胶原酶与气性坏疽中的细菌入侵有关。从组织中进行细胞的有效释放需要胶原酶和中性蛋白酶的作用。每摩尔的胶原酶可被四克的钙原子（Ca 2+ ）所激活。培养滤液中被认为至少含有7种分子量在68-130kDa之间的不同蛋白酶。最适pH为6.3-6.8。该酶通常用于消化组织样品中的结缔成分以释放单个细胞。胶原酶处理可引起某些细胞的死亡。通常，使用0.1至5 mg/mL的浓度用于消化。反应时间可从15分钟至数小时，并在不引起过多细胞死亡的情况下达到令人满意的细胞解离效果。优选含有钙和BSA的Krebs Ringer缓冲液，而且Zn 2+ 是活性所必需的。每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。

0.5-5.0 FALGPA U/mg solid, ≥125 CDU/mg solid

天然

溶组织梭菌(Clostridium histolyticum)