壳聚糖酶 来源于灰色链霉菌

C755131-10U

10U

lyophilized powder, ≥50 units/mg protein (Bradford)

Sigma的酶已被用于水解卵菌根腐丝囊霉Aphanomyces euteiches（豆科植物的主要寄生虫）的细胞壁粗制品。它还被用于完全脱-N-乙酰化壳聚糖的酶促水解，以便在制备生物相容性壳聚糖-藻酸盐凝胶时得到壳聚糖低聚物混合物。来自灰链霉菌的壳聚糖酶已被用于消化泪腺（LG）组织。来自灰色链霉菌（Streptomyces griseus）的壳聚糖酶已被用于评估几丁质来源对几丁质酶和壳聚糖酶产生的影响。来自灰色链霉菌（Streptomyces griseus）的壳聚糖酶还被用于研究：通过使用胶体几丁质和不同程度的壳聚糖脱乙酰化来研究灰色链霉菌（Streptomyces griseus）HUT 6037的几丁质酶和壳聚糖酶的有效产生。应用： 壳聚糖酶作为细胞外分泌酶由灰链霉菌 合成，其分子量为34 kDa。它的最适温度和pH分别为60°C和5.7。壳聚糖酶属于糖基水解酶家族，并包含α-螺旋三级结构，类似于溶菌酶，在两瓣之间存在与底物结合的裂隙。 Chitosanase is synthesized from Streptomyces griseus as an extracellular secreted enzyme and corresponds to a molecular weight of 34 kDa. It has a temperature and pH optimum of 60°C and 5.7, respectively. Chitosanase belongs to the glycosyl hydrolase family and comprises α-helical tertiary structure similar to lysozyme with substrate-binding cleft placed between two lobes. Application: Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan. Chitosanase from Streptomyces griseus has been used in the digestion of lacrimal gland (LG) tissues. The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches , a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.

壳聚糖酶可在N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖残基之间的β（1,4）键处催化N-乙酰化壳聚糖的内水解。来自灰链霉菌的壳聚糖酶能够水解壳聚糖和羧甲基纤维素。壳聚糖酶存在于多种类型的微生物中 ，并可用于真菌细胞壁的裂解。