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名称

软骨素酶AC 来源于肝素黄杆菌

货号

C755194-10U

包装规格

10U

级别

重组

浓度

expressed in E. coli,≥200 units/mg protein, For Chondroitin Sulfate Analysis

产品介绍

已经过高度纯化以去除干扰性β-葡糖醛酸糖苷酶和蛋白酶活性，可用于硫酸软骨素水解和 HPLC 分析。来自肝素黄杆菌的软骨素酶AC是一种通过消除机制将己糖胺和葡萄糖醛酸残基之间具有（1-4）键的硫酸化和非硫酸化多糖链裂解的酶。所得到的寡糖产物主要是具有不饱和糖醛酸的二糖。软骨素酶AC可特异性地降解硫酸软骨素A和C，但不可降解硫酸软骨素B（硫酸皮肤素）。软骨素酶AC已用于商业样品（例如膳食补充剂）中硫酸软骨素的分析。 Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate). Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements. Highly purified to remove interferring β-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis.

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