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Product details

名称

软骨素酶 ABC 来源于普通变形杆菌

货号

C755222-10U

包装规格

10U

浓度

BSA free, lyophilized powder, specific activity 50-250 units/mg protein

产品介绍

该酶已被用于研究采用成年大鼠脑细胞，硫酸软骨素蛋白多糖所诱导的发急性、长期变化的后果。以新鲜牛角膜基质为原料，采用软骨素酶ABC对氨基聚糖进行酶解，测定了其在角质细胞培养中的含量。 The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae.

生化机理

硫酸软骨素酶ABC催化含有(1-4)-β-D-己糖胺基和(1-3)-β-葡聚糖或(1-3)-α-葡聚糖-L-iduronosyl与含有4-脱氧-β-D-葡聚糖-4-enuronosyl基团的双糖链接的清除性降解。它作用于4-硫酸软骨素、6-硫酸软骨素和皮肤硫酸软骨素，缓慢作用于透明质酸盐。分子量约为120 kDa，含有2个不相同的亚基，分子量分别为86 kDa和32 kDa。找到的最佳pH值与硫酸软骨素为8.0，与透明质酸为6.8，以及最佳温度是37°C。以1 mM Zn 2+ 为抑制剂，0.05 M醋酸盐为酶的激活剂。

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