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名称
α-糜蛋白酶原A 来源于牛胰腺
别名
A-胰凝乳蛋白酶原
英文别名
chymotrypsin A zymogen | Chymotrypsinogen | Chymotrypsinogen A
货号
C755332-100mg
包装规格
100mg
级别
BioReagent
浓度
After activation to chymotrypsin ≥40 units/mg powder
产品介绍
来自牛胰腺的胰凝乳蛋白酶原是一种含有5个二硫键的酶原。它的等电点是8.97。 一种丝氨酸蛋白酶,水解肽键与肽键羧基末端的芳香族或大型疏水侧链(Tyr、Trp、Phe 、Met)的肽键。 处理说明: 胰凝乳蛋白酶原A需要有限的蛋白水解才能激活。胰凝乳蛋白酶原A可由胰蛋白酶和胰凝乳蛋白酶激活(自溶激活)以形成m α、β、γ、δ和π胰凝乳蛋白酶(取决于激活条件)。胰凝乳蛋白酶原是一种蛋白酶,优先裂解芳香族氨基酸(包括色氨酸、酪氨酸和苯丙氨酸)羧基侧的肽。它也水解亮氨酸、蛋氨酸和丙氨酸羧基侧的肽。 产品应用 1、作为蛋白质模型用于结晶重现性研究。 2、用于质谱研究前α-麦醇溶蛋白的水解。 注:活化为硝酸凝乳蛋白酶后,在pH7.8、25℃条件下,1个单位将每分钟水解1.0 μmol BTEE
生化机理
胰凝乳蛋白酶原A需要有限的蛋白水解才能激活。胰凝乳蛋白酶原A可由胰蛋白酶和胰凝乳蛋白酶激活(自溶激活)以形成 m α、β、γ、δ和π胰凝乳蛋白酶(取决于激活条件)。 胰凝乳蛋白酶原是一种蛋白酶,优先裂解芳香族氨基酸(包括色氨酸、酪氨酸和苯丙氨酸)羧基侧的肽。它也水解亮氨酸、蛋氨酸和丙氨酸羧基侧的肽。一种丝氨酸蛋白酶,水解肽键与肽键羧基末端的芳香族或大型疏水侧链 (Tyr、Trp、Phe、Met) 的肽键。
生物活性
After activation to chymotrypsin ≥40 units/mg powder; One unit will hydrolyze 1.0 micromole of N-Benzoyl-L-Tyrosine Ethyl Ester (BTEE) per minute at pH7.8 at 25 ℃.
来源
天然
种属
牛(Bovine)
Safety
「No Dangerous Attributes」
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Research chemical 100mg

item number:C755332-100mg
Product model:100mg
level: BioReagent
Lead Time:30days
sold 276 Items
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