腔肠素400a是生色团，其在Ca | 2+ |存在下产生由水母发光蛋白显示的化学发光。水母发光蛋白被用作细胞内Ca | 2+ |的化学发光探针，由各种腔肠素衍生物生成的这种蛋白质的衍生物产生了一系列具有不同光谱特性的Ca | 2+ |探针。腔肠素400a是腔肠素的双脱氧衍生物。在通过海肾荧光素酶（Rluc）转化后，腔肠素400a的最大发射波长为395nm。腔肠素400 a通常与1类和3类GFP受体配对。 product description： Coelenterazine 400 a is a chromophore that produces the chemiluminescence displayed by the Aequorin protein in the presence of Ca|2+|. Aequorin is employed as a chemiluminescent probe for intracellular Ca|2+|, and derivatives of this protein generated with various Coelenterazine derivatives produced a range of Ca|2+|probes with varying spectral properties. Coelenterazine 400 a is a bisdeoxy derivative of coelenterazine. Coelenterazine 400 a displays an emission maximum of 395nm following conversion by Renilla luciferase (Rluc). Coelenterazine 400 a is commonly paired with class 1 and 3 GFP acceptors. Applications： Coelenterazine 400 a is a blue emitting Rluc substrate for bioluminescence resonance energy transfer 2 (BRET2) studies.