BODIPY-cholesterol 是一种带有荧光 BODIPY 基团的亲脂性和细胞通透性胆固醇类似物。BODIPY-cholesterol 可用于监测细胞内固醇摄取和细胞器间固醇通量。(激发/发射波长: 480/508 nm)。 Biological activity： BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm) In vitro research： BODIPY-cholesterol assay To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 μM), mixed thoroughly and incubated for 10 -20 mins at 37 °C The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment