D-荧光素已用于制备发光测量法测定氮氧化物用的反应混合物。它还被用于制备荧光素检测用D-荧光素储备溶液。Km 约为 2 μM 的萤火虫荧光素酶的底物。应用： D-荧光素主要由发光生物生产。它可以简便地绕过血睾屏障、血脑屏障和血胎盘屏障进行循环。这种小分子对光线和氧气敏感。其粉末形式也对水分敏感。 D-Luciferin is mainly produced in bioluminescent organisms. It has the ability to simply circulate across the blood testis barrier, blood brain barrier and the blood placenta barrier. This small molecule is sensitive to light and oxygen. It is also sensitive to moisture in its powder form. Application: D-Luciferin has been used in the preparation of the reaction mixture for nitric oxide determination by minescence measurement. It has also been used in the preparation of D-luciferin stock solution for luciferin assays. Substrate for firefly luciferase with a Km of approx 2 μM.