DyLight™ 550 Maleimide

DyLight™ 550 马来酰亚胺

DyLight™ 550 Maleimide

D638915-1mg

1mg

-20°C储存,干燥

超低温冰袋运输

Product introduction： DyLight 550 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 550 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes. provides vibrant orange-to-red fluorescence with better performance than other rhodamine derivatives including Alexa Fluor™ 555, TRITC and Cy3™ dye for fluorescent applications over a broad pH range (pH 4 - 9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. Features of DyLight 550 Maleimide: • High performance— DyLight 550 shows brighter fluorescence than Alexa Fluor 555, TRITC and Cy3 dye • Specific— maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH) • Efficient labeling methods— well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling • Optimized antibody labeling procedure— complete protocol for IgG reduction and labeling and calculating the labeling efficiency Applications: • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies DyLight 550 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more aboutmaleimide chemistry.

DMF(Dimethylformamide)

562

576

Thiol