DJ001

DJ001

D651930-5mg

5mg

≥99%

-20°C储存

超低温冰袋运输

DJ001 is a highly specific, selective and non-competitive protein tyrosine phosphatase-σ (PTPσ) inhibitor with an IC 50 of 1.43 μM. DJ001 displays no inhibitory activity against other phosphatases, with only modest inhibitory activity against Protein Phosphatase 5. DJ001 promotes promote hematopoietic stem cell regeneration In Vitro DJ001 (5-1000 ng/mL; 3-7 days; BM KSL cells) treatment increases the percentages and numbers of BM KSL cells in culture compared with control cultures. DJ001 treatment also significantly increases the numbers of colony forming cells (CFCs) in 3 day culture of BM KSL cells. The BM KSL cells are irradiated with 300 cGy and placed in media (containing 20 ng/mL Thrombopoietin, 100 ng/mL stem cell factor (SCF), 50 ng/mL Flt3 ligand, TSF) with and without 1 μg/mL DJ001 for 3 days. DJ001 treatment increases recovery of BM CFCs and multipotent colony-forming unit–granulocyte erythroid monocyte megakaryocyte (CFU-GEMM) colonies compared with control cultures. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: BM KSL cells Concentration: 5 ng/mL, 10 ng/mL, 100 ng/mL, 1000 ng/mL Incubation Time: 3 days or 7 days Result: Increased the percentages and numbers of BM KSL cells. Also significantly increased the numbers of colony forming cells (CFCs) in 3 day culture of BM KSL cells. In Vivo DJ001 (5 mg/kg; subcutaneous injection; for 24 hours; female C57BL/6 mice) significantly decreases the percentage of apoptotic BM KSL cells in C57BL/6 mice at 24 h following 500 cGy TBI. DJ001 suppresses radiation-induced HSC apoptosis via activation of the RhoGTPase, RAC1, and induction of BCL-X L . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Female C57BL/6 mice (8-10-week-old) irradiated with total body irradiation (TBI) Dosage: 5 mg/kg Administration: Subcutaneous injection; for 24 hours Result: Significantly decreased the percentage of apoptotic BM KSL cells in C57BL/6 mice at 24 h following 500 cGy TBI. Form:Solid IC50& Target:IC50: 1.43 μM (Protein tyrosine phosphatase-σ)

DJ001 是一种高度特异性、选择性和非竞争性的蛋白酪氨酸磷酸酶-σ（PTPσ）抑制剂，IC 50 为 1.43 μM。DJ001 对其他磷酸酶没有抑制活性，对蛋白磷酸酶（PTP）只有适度的抑制活性。

2161305-12-8

C15H12N2O3

268.27 g/mol

44161060

C1=CC=C(C=C1)C(=O)/C=C\NC2=CC(=CC=C2)[N+](=O)[O-]

(Z)-3-(3-nitroanilino)-1-phenylprop-2-en-1-one

1S/C15H12N2O3/c18-15(12-5-2-1-3-6-12)9-10-16-13-7-4-8-14(11-13)17(19)20/h1-11,16H/b10-9-

OVTJWWUGQKIRCL-KTKRTIGZSA-N

C1=CC=C(C=C1)C(=O)C=CNC2=CC(=CC=C2)[N+](=O)[O-]

44161060

DMSO : 250 mg/mL (931.90 mM; Need ultrasonic)