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名称
DNA Ladder (50-500bp)
别名
DNA电泳分子量标准(50-500bp) | DNA分子量标准 Marker (50-500 bp)
英文别名
50 bp-ⅠDNA ladder | 50 bp DNA Ladder Marker | 50 bp DNA Step Ladder | 50 bp DNA Marker
英文名称
DNA Ladder (50-500bp)
货号
D745352-100T
包装规格
100T
级别
适用于电泳
浓度
50-500bp, 9 bands, Blue, 50bp/ 100bp/ 150bp/ 200bp/ 250bp/ 300bp/ 350bp/ 400bp/ 500bp
储存温度
-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
稳定性与储存
Store at room temperature short term (3-6 months). Store at 4℃ long term (24 months). Store at -20℃ long term (60 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
产品介绍
储存缓冲液:10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6 产品简介 本产品是由9条线状双链 DNA 片段组成,保存于1xLoading Buffer中,条带密集,需用高浓度胶低电压电泳,适用于较精确确认DNA片段大小。大小范围为 50-500bp,分别为 50bp、100bp、150bp、200bp、250bp、300bp、350bp、400bp、500bp;250 bp为加亮带,浓度为其他条带的2.5倍;5 ul产品中,每条带含量约40 ng,加亮带约100 ng。 使用建议 1. 建议点样量5 ul,宽胶孔适当增加上样量。 2. 建议用2.5-3.5 % Agarose,或5 %PAGE胶,电压4-10 v/cm,0.5xTBE缓冲液电泳。注意及时更换电泳缓冲液并使用新制备的凝胶,以达到理想的结果。 3. 通过EB进行染色,或者用其他的核酸染料进行染色,在紫外灯下观察电泳条带。 注意事项 1. 本产品已保存在1xLoading Buffer中,可直接进行电泳,使用方便,电泳图像清晰。 2. 使用含有EB的琼脂糖凝胶进行电泳检测时,将溴酚蓝条带电泳到约2/3的距离时即可,否则小片段部分由于EB脱离DNA,会导致条带变暗。 3. 随附 5xLoading buffer 可用于检测样品时使用。 5x Loading Buffer 成分: 10 mM Tris-HCl,5 mM EDTA,pH7.6,0.03 %溴酚蓝,0.03 %二甲苯青,30 % 甘油 组分表 D745352
pH
8.0-8.5
Safety
「No Dangerous Attributes」
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Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:D745352-100T
Product model:100T
level: Suitable for electrophoresis
Lead Time:30days
sold 549 Items
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100T

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