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名称
DNA Ladder (100-15000bp)
别名
DNA Marker DL15000 | DNA电泳分子量标准R(100-15000bp) | DNA分子量标准 Marker (100-15000 bp)
英文别名
15kb DNA Ladder | 15kb DNA Marker | DNA Marker (100-15000 bp) | D15000 DNA Marker(100-15000bp) | DNA Marker DL15000
英文名称
DNA Ladder (100-15000bp)
货号
D745353-100T
包装规格
100T
级别
适用于电泳
浓度
100-15000bp, 11 bands, Blue, 100bp/ 250bp/ 500bp/ 750bp/ 1000bp/ 1500bp/ 2000bp/ 3000bp/ 5000bp/ 8000bp/ 15000bp
储存温度
-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
稳定性与储存
Store at room temperature short term (3-6 months). Store at 4℃ long term (24 months). Store at -20℃ long term (60 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
产品介绍
储存缓冲液:10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6 产品说明 1. 本产品是由11条线状双链DNA片段组成,大小范围为100bp~15kb,分别为100bp,250bp,500bp,750bp,1000bp, 1500bp,2000bp,3000bp,5000bp,8000bp,15000bp。500bp和1500bp为加亮带指示,浓度为其它条带的2.5倍,便于电泳后观察。 2. 5ul本产品中,常规条带的含量约为30ng,加亮带含量约75ng。 3. 本产品已保存在1xLoading Buffer 中,可直接用于电泳,使用方便。 4. 本产品不能用于聚丙烯酰胺凝胶电泳。 推荐电泳缓冲液及琼脂糖凝胶浓度: 本产品推荐使用1xTAE电泳缓冲液,推荐琼脂糖浓度: 1.0%~1.5%,小片段电泳,建议使用GelRed核酸染料。 使用说明 1. 制备含核酸染料的琼脂糖凝胶,如EB或者GelRed。 2. 凝胶浓度对DNA电泳的影响比较大,本品建议的琼脂糖凝胶浓度为 1.0%~1.5%。 3. 建议使用1xTAE缓冲液,电泳电压不超过10v/cm。 4. 一般常见的3.5mm加样孔,建议用量3~5ul,宽胶孔适当增加上样量。 5. 电泳至合适距离:对于EB,Goldview,GRBlue等核酸染料,一般电泳时溴酚蓝条带的位置不要超过凝胶三分之二,否则,小片段会由于核酸染料与DNA脱离而变弱。对于GelRed等核酸染料,可以电泳更长的距离,只要最小片段不要跑出凝胶即可。一般溴酚蓝指示带距凝胶边缘不小于1cm。 6. 电泳结束后,在紫外灯下观察电泳条带。 7. 产品内所附的5x Loading buffer 用于与待检测样品混合后点样使用,含溴酚蓝和二甲苯青双指示剂。 组分表 D745353
pH
8.0-8.5
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Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:D745353-100T
Product model:100T
level: Suitable for electrophoresis
Lead Time:30days
sold 313 Items
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