去乙酰化酶抑制剂混合物

D752122-1ml

1ml

100X

阿拉丁的去乙酰化酶抑制剂混合物(100X)(Deacetylase Inhibitor Cocktail, 100X)是一种用于维持组蛋白和其它非组蛋白乙酰化状态的去乙酰化酶抑制剂混合物(40μM Trichostatin A, 1mM EX-527, 400mM nicotinamide and 200mM sodium butyrate in 70% DMSO)。细胞或组织等提取物中含有许多内源性的蛋白酶、磷酸酶、去乙酰化酶等，容易导致提取物中的蛋白降解或去修饰，从而影响后续的蛋白检测。因此在提取物中添加适当的蛋白酶、磷酸酶、去乙酰化酶等抑制剂是防止蛋白降解和去修饰的有效方法。为更好地检测细胞内蛋白的乙酰化水平，可在细胞培养液中添加去乙酰化酶抑制剂一段时间后再收集细胞。本去乙酰化酶抑制剂混合物可以有效抑制各类去乙酰化酶(包括class I/II/III HDAC)对蛋白的去乙酰化作用，保持蛋白乙酰化状态。本去乙酰化酶抑制剂混合物含40μM Trichostatin A, 1mM EX-527, 400mM nicotinamide 和200mM sodium butyrate，配制在含适量水的DMSO中。本产品使用便捷，通常以1:100的比例把去乙酰化酶抑制剂混合物加入裂解液或细胞培养液中即可直接使用。阿拉丁针对不同类型样品的蛋白提取，提供了不同类型的蛋白酶、磷酸酶、去乙酰化酶等抑制剂混合物以供选择，具体请参考附表1。本产品可以有效抑制各种去乙酰化酶，包括class I/II/III去乙酰化酶等 。曲古菌素A(Trichostatin A)是class I/II 去乙酰化酶可逆抑制剂；EX-527是SIRT1的选择性可逆抑制剂，对SIRT2和 SIRT3也有一定的抑制作用；烟酰胺(nicotinamide)是class III 去乙酰化酶(Sirtuins，即SIRT1-7)可逆抑制剂；丁酸钠(sodium butyrate)是class I/II 去乙酰化酶可逆抑制剂。各种抑制剂的具体信息请参考附表2。蛋白酶、磷酸酶和去乙酰化酶抑制剂混合物的比较和选择请见：https://www.aladdin-e.com/。一个包装的本产品通常足够用于100ml的裂解液或细胞培养液的配制。 注意事项 ： 本产品可以适当分装后保存，并应在临使用前添加到裂解液等适当溶液中。本产品中含有的去乙酰化酶抑制剂对人体有害，操作时请小心，并注意有效防护以避免直接接触人体或吸入体内。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 ： 1.本去乙酰化酶抑制剂混合物为100X的储存液，使用时按照1:100的比例加入到裂解液或培养液中(例如，1ml裂解液或培养液中加入10μl去乙酰化酶抑制剂混合物)，混匀后即可使用。含有去乙酰化酶抑制剂混合物的裂解液或培养液宜现用现配，不宜配制后冻存待后续使用。附表1. 阿拉丁提供的不同类型的蛋白提取用蛋白酶、磷酸酶、去乙酰化酶等抑制剂混合物：产品编号产品名称包装P1005蛋白酶抑制剂混合物(通用型, 100X) 1mlP1006蛋白酶抑制剂混合物(通用型, 100X) 5mlP1008蛋白酶抑制剂混合物(通用型, 质谱兼容, 50X) 1mlP1009蛋白酶抑制剂混合物(通用型, 质谱兼容, 50X) 5mlP1010蛋白酶抑制剂混合物(哺乳动物样品抽提用, 100X) 1mlP1011蛋白酶抑制剂混合物(哺乳动物样品抽提用, 100X) 5mlP1015蛋白酶抑制剂混合物(植物样品抽提用, 100X) 1mlP1016蛋白酶抑制剂混合物(植物样品抽提用, 100X) 5mlP1020蛋白酶抑制剂混合物(真菌或酵母抽提用, 100X) 1mlP1021蛋白酶抑制剂混合物(真菌或酵母抽提用, 100X) 5mlP1025蛋白酶抑制剂混合物(细菌抽提用, 100X) 1mlP1026蛋白酶抑制剂混合物(细菌抽提用, 100X) 5mlP1030蛋白酶抑制剂混合物(His-Tag蛋白纯化用, 100X) 1mlP1031蛋白酶抑制剂混合物(His-Tag蛋白纯化用, 100X) 5mlP1045蛋白酶磷酸酶抑制剂混合物(通用型, 50X) 各2mlP1046蛋白酶磷酸酶抑制剂混合物(通用型, 50X) 各10mlP1048蛋白酶磷酸酶抑制剂混合物(通用型, 质谱兼容, 50X) 各1mlP1049蛋白酶磷酸酶抑制剂混合物(通用型, 质谱兼容, 50X) 各5mlP1050蛋白酶磷酸酶抑制剂混合物(哺乳动物样品抽提用, 50X) 各2mlP1051蛋白酶磷酸酶抑制剂混合物(哺乳动物样品抽提用, 50X) 各10mlP1055蛋白酶磷酸酶抑制剂混合物(植物样品抽提用, 50X) 各2mlP1056蛋白酶磷酸酶抑制剂混合物(植物样品抽提用, 50X) 各10mlP1060蛋白酶磷酸酶抑制剂混合物(真菌或酵母抽提用, 50X) 各2mlP1061蛋白酶磷酸酶抑制剂混合物(真菌或酵母抽提用, 50X) 各10mlP1065蛋白酶磷酸酶抑制剂混合物(细菌抽提用, 50X) 各2mlP1066蛋白酶磷酸酶抑制剂混合物(细菌抽提用, 50X) 各10mlP1081磷酸酶抑制剂混合物A (50X) 2mlP1082磷酸酶抑制剂混合物A (50X) 10mlP1086磷酸酶抑制剂混合物B (50X) 2mlP1087磷酸酶抑制剂混合物B (50X) 10mlP1091磷酸酶抑制剂混合物C (50X) 2mlP1092磷酸酶抑制剂混合物C (50X) 10mlP1096磷酸酶抑制剂混合物D (50X) 2mlP1097磷酸酶抑制剂混合物D (50X) 10mlP1112去乙酰化酶抑制剂混合物(100X) 1mlP1113去乙酰化酶抑制剂混合物(100X) 5ml附表2. 常用蛋白酶、磷酸酶、去乙酰化酶抑制剂的具体相关信息：名称分子量目标酶家族抑制类型AEBSF239.5丝氨酸蛋白酶不可逆Aprotinin6511.5丝氨酸蛋白酶可逆Bestatin308.38氨基肽酶可逆E64357.4半胱氨酸蛋白酶不可逆Leupeptin475.6丝氨酸和半胱氨酸蛋白酶可逆Pepstatin A685.9天冬氨酸蛋白酶可逆EDTA372.24金属蛋白酶可逆Phenanthroline198.22金属蛋白酶可逆Phosphoramidon587.5金属蛋白酶可逆Sodium fluoride41.99酸性磷酸酶可逆Sodium pyrophosphate221.9丝氨酸/苏氨酸磷酸酶不可逆β-glycerophosphate306.11丝氨酸/苏氨酸磷酸酶可逆Imidazole68.2碱性磷酸酶可逆Sodium molybdate205.9酸性磷酸酶不可逆Sodium tartrate dihydrate230.1酸性磷酸酶可逆Sodium orthovanadate183.91碱性和酪氨酸磷酸酶可逆(-)-p-Bromotetramisole oxalate373.22碱性磷酸酶不可逆Cantharidin196.20蛋白磷酸酶2A可逆Microcystin-LR995.17蛋白磷酸酶1和蛋白磷酸酶2A可逆Calyculin A1009.17蛋白磷酸酶1和蛋白磷酸酶2A可逆Trichostatin A302.37class I/II HDAC可逆EX-527248.71SIRT1 over SIRT2 & SIRT3可逆Nicotinamide122.12class III HDAC可逆Sodium butyrate110.09class I/II HDAC可逆 Aladdin's Deacetylase Inhibitor Cocktail, 100X is designed to maintain the acetylation of histones and other non-histone proteins. It contains 40μM Trichostatin A, 1mM EX-527, 400mM nicotinamide, and 200mM sodium butyrate in 70% DMSO.Cell or tissue extracts contain many endogenous proteases, phosphatases, and deacetylases, which easily leads to degradation or de-modification of protein samples. Therefore, adding appropriate inhibitors is generally necessary for protein extraction. To better detect the acetylation level of intracellular proteins, appropriate deacetylase inhibitors should be added to the cell culture for a period of time before collecting cells for protein extraction. This product can effectively inhibit various deacetylases including class I/II/III HDAC and maintain the acetylation state of proteins.For the protein extraction from different types of samples, aladdin provides a wide variety of protease, phosphatase, deacetylase and other inhibitor cocktails for selection. Please refer to Table 1 in Appendix for details.This product can effectively inhibit various deacetylases, including class I/II/III deacetylases. Among its components, Trichostatin A is a reversible inhibitor of class I/II deacetylase. EX-527 is a selective and reversible inhibitor of SIRT1 and also has a certain inhibitory effect on SIRT2 and SIRT3. Nicotinamide is a reversible inhibitor of Class III deacetylase (Sirtuins, SIRT1-7). Sodium butyrate is a reversible inhibitor of class I/II deacetylase. Please refer to Table 2 in Appendix for specific information of various inhibitors.This product is usually sufficient for preparing 100ml of lysis buffer or cell culture medium. Precautions ： It is recommended to store this product in aliquot. This product should be added into solutions immediately before use.The deacetylase inhibitors contained in this product are harmful to human. Please take effective measures to avoid direct contact or inhalation.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Appendix：Table 1. Inhibitor cocktails from .Cat. No.Product NamePack SizeP1005Protease Inhibitor Cocktail for General Use, 100X1mlP1006Protease Inhibitor Cocktail for General Use, 100X5mlP1008Protease inhibitor cocktail for general use, MS-SAFE, 50X1mlP1009Protease inhibitor cocktail for general use, MS-SAFE, 50X5mlP1010Protease inhibitor cocktail for mammalian cell and tissue extracts, 100X1mlP1011Protease inhibitor cocktail for mammalian cell and tissue extracts, 100X5mlP1015Protease inhibitor cocktail for plant cell and tissue extracts, 100X1mlP1016Protease inhibitor cocktail for plant cell and tissue extracts, 100X5mlP1020Protease inhibitor cocktail for fungal and yeast extracts, 100X1mlP1021Protease inhibitor cocktail for fungal and yeast extracts, 100X5mlP1025Protease inhibitor cocktail for bacterial cell extracts, 100X1mlP1026Protease inhibitor cocktail for bacterial cell extracts, 100X5mlP1030Protease inhibitor cocktail for purification of His-tagged proteins, 100X1mlP1031Protease inhibitor cocktail for purification of His-tagged proteins, 100X5mlP1045Protease and phosphatase inhibitor cocktail for general use, 50X2ml eachP1046Protease and phosphatase inhibitor cocktail for general use, 50X10ml eachP1048Protease and phosphatase inhibitor cocktail for general use, MS-safe, 50X1ml eachP1049Protease and phosphatase inhibitor cocktail for general use, MS-safe, 50X5ml eachP1050Protease and phosphatase inhibitor cocktail for mammalian cell and tissue extracts, 50X2ml eachP1051Protease and phosphatase inhibitor cocktail for mammalian cell and tissue extracts, 50X10ml eachP1055Protease and phosphatase inhibitor cocktail for plant cell and tissue extracts, 50X2ml eachP1056Protease and phosphatase inhibitor cocktail for plant cell and tissue extracts, 50X10ml eachP1060Protease and phosphatase inhibitor cocktail for fungal and yeast extracts, 50X2ml eachP1061Protease and phosphatase inhibitor cocktail for fungal and yeast extracts, 50X10ml eachP1065Protease and phosphatase inhibitor cocktail for bacterial cell extracts, 50X2ml eachP1066Protease and phosphatase inhibitor cocktail for bacterial cell extracts, 50X10ml eachP1081Phosphatase inhibitor cocktail A, 50X2mlP1082Phosphatase inhibitor cocktail A, 50X10mlP1086Phosphatase inhibitor cocktail B ,50X2mlP1087Phosphatase inhibitor cocktail B ,50X10mlP1091Phosphatase inhibitor cocktail C ,50X2mlP1092Phosphatase inhibitor cocktail C ,50X10mlP1096Phosphatase inhibitor cocktail D ,50X2mlP1097Phosphatase inhibitor cocktail D ,50X10mlP1112Deacetylase Inhibitor Cocktail,100X1mlP1113Deacetylase Inhibitor Cocktail,100X5mlTable 2. Information of commonly used protease, phosphatase, and deacetylase inhibitors. NameMW Target EnzymeInhibition AEBSF239.5Serine proteaseirreversibleAprotinin6511.5Serine proteaseReversibleBestatin308.38AminopeptidaseReversibleE64357.4Cysteine proteaseirreversibleLeupeptin475.6Serine and Cysteine proteaseReversiblePepstatin A685.9Aspartic proteaseReversibleEDTA372.24MetalloproteinaseReversiblePhenanthroline198.22MetalloproteinaseReversiblePhosphoramidon587.5MetalloproteinaseReversibleSodium fluoride41.99Acid phosphataseReversibleSodium pyrophosphate221.9Serine/Threonine Phosphataseirreversibleβ-glycerophosphate306.11Serine/Threonine PhosphataseReversibleImidazole68.2Alkaline phosphataseReversibleSodium molybdate205.9Acid phosphataseirreversibleSodium tartrate 230.1Acid phosphataseReversibleSodium orthovanadate183.91Alkaline and Tyrosine PhosphatasesReversible(-)-p-Bromotetramisole373.22Alkaline phosphataseirreversibleCantharidin196.20Protein Phosphatase 2AReversibleMicrocystin-LR995.17Protein Phosphatase 1 and Protein Phosphatase 2AReversibleCalyculin A1009.17Protein Phosphatase 1 and Protein Phosphatase 2AReversibleTrichostatin A302.37class I/II HDACReversibleEX-527248.71SIRT1 over SIRT2 & SIRT3ReversibleNicotinamide122.12class III HDACReversibleSodium butyrate110.09class I/II HDACReversible Instructions for Use ： 1. This product is a 100X concentrated stock solution and should be added to the lysis buffer or culture medium at 1:100 before use (e.g., add 10μl of deacetylase inhibitor cocktail to 1ml of lysis buffer). The lysis buffer or culture medium containing the deacetylase inhibitors should be prepared freshly. After preparation, protein extraction from cells or tissues should be performed immediately.