双色非变性PAGE蛋白上样缓冲液

D752144-2ml

2ml

5X, 无气味

阿拉丁生产的双色非变性PAGE蛋白上样缓冲液(5X, 无气味) (Dual Color Non-denatured Gel Protein Sample Loading Buffer, 5X, Odorless)，是一种经过改良的更加安全健康的无气味的使用蓝色和红色染料、5倍浓缩的蛋白上样缓冲液。 本产品更加安全健康。本产品使用了无气味、水溶性更稳定、还原能力相近的还原剂替代了有气味的二硫苏糖醇(DTT)或巯基乙醇(2-Mercaptoethanol)，从而可以确保本双色非变性PAGE蛋白上样缓冲液在正常使用时不会有异味，使蛋白上样操作更加安全健康。 除了没有气味外和染料不同外，本产品和常规的非变性蛋白上样缓冲液的使用效果一致。本产品可以用于常规的非变性PAGE蛋白样品的上样。除了没有异味和染料不同外，本产品与常规非变性PAGE蛋白上样缓冲液的使用效果一致，未观察到有任何显著差异。 本产品可以用于转膜后的泳道示踪：本双色非变性PAGE蛋白上样缓冲液(5X, 无气味)中含特殊的红色染料，该红色染料可随蛋白转印到PVDF或NC等印迹膜上，从而可以用于泳道位置的指示，便于剪膜等操作。双色类SDS-PAGE蛋白上样缓冲液产品的电泳及转膜效果示意图参见图1。 图1. 双色类SDS-PAGE蛋白上样缓冲液产品用于UltraBio™ Plus PAGE预制胶(Tris-Gly, 12%, 10孔) 的电泳后的凝胶图(Gel after electrophoresis)、转膜后PVDF膜图(Blot)和转膜后凝胶图(Gel after transfer)。 注意事项 ： 本产品用于蛋白变性时，建议95℃水浴或PCR仪加热5分钟，温度过高(如100℃)或时间过长(如超过15分钟)，有可能会导致蛋白降解或上样缓冲液中指示剂的颜色异常。 本产品不含剧毒的巯基乙醇和有刺激性气味的DTT，但还原效果一致，对于蛋白样品的处理效果和电泳效果一致。 本产品必须完全溶解后再使用。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 ： 1.在室温或不超过37℃的水浴中溶解双色非变性PAGE蛋白上样缓冲液(5X, 无气味)。水浴溶解后立即室温存放，尽量避免长时间置于水浴中。使用完毕后置于-20℃保存。2.按照每4微升蛋白样品加入1微升双色非变性PAGE蛋白上样缓冲液(5X, 无气味)的比例，混合蛋白样品和双色非变性PAGE蛋白上样缓冲液(5X, 无气味)。注：本产品稀释至1X后也可以直接用于细胞或组织样品的裂解。3.通常电泳至红色或蓝色染料到达胶的底端处附近即可停止电泳。 Aladdin's Dual Color Non-denaturing Protein Sample Loading Buffer (5X, Odorless) is an improved protein loading buffer with red and blue dyes, which can be used to load protein samples onto a non-denaturing PAGE gel for protein resolution.This product is safe to use. Instead of odoriferous DTT or 2-Mercaptoethanol reducing agent, this product employs an odorless and more stable reducing agent with similar reducing capability.This product has the same performance as conventional non-denaturing protein loading buffers.This product can be used for lane tracing after membrane transfer. It contains a special red dye which can be transferred to a PVDF or NC membrane to indicate the position of the lane, facilitating operations such as film cutting. Please refer to Figure 1 for the electrophoresis and the transfer results of this product.Figure 1. Aladdin's Dual Color Non-denaturing Protein Sample Loading Buffer (5X, Odorless) in the UltraBio™ Plus PAGE Precast Gel (Tris-Gly, 12%, 10 wells) after electrophoresis and transfer, as well as in the PVDF membrane after transfer (Blot). Precautions ： This product must be completely dissolved and mixed well before use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use ： 1. Thaw this product at room temperature or in a water bath no more than 37℃. Place at room temperature immediately once dissolved and store at -20℃ after use.2. Add 1μl of this product per 4 μl of protein sample, and mix well. The mixture can be directly loaded to a gel.Note: After dilution to 1X, this product can also be used for sample lysis directly.3. Stop electrophoresis when the red or blue dye reaches the bottom of the gel.