脱氧核糖核酸酶 I 来源于牛胰腺

D755014-15KU

15KU

Type IV, lyophilized powder,≥2,000 Kunitz units/mg protein

DNAse I可用于切开DNA，作为将标记碱基插入DNA的第一步。来自sigma的该酶已用于处理大鼠的脑组织。该研究表明星形胶质细胞的轴突生长不受少突胶质细胞抑制。在另一项研究中，大肠杆菌的解冻固定样本被来自Sigma的DNAseI以及其他酶进行消化。消化是在进行通透化处理和核酸染色前完成的。来自牛胰腺的脱氧核糖核酸酶I已用于研究枯草芽孢杆菌中核酸酶的二维酶谱分析。来自牛胰腺的脱氧核糖核酸酶I还用于研究小沟和大沟结合药物和嵌入剂对小沟结合蛋白RecA和脱氧核糖核酸酶I的DNA结合的影响。用于从蛋白质样品中除去 DNA。 DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the processing of rat brain tissue. This study showed that axonal growth on astrocytes is not inhibited by oligodendrocytes. In another study, thawed fixed samples of E. coli were digested with DNAse I from Sigma along with other enzymes. The digestion was done before permeabilization and staining of the nucleic acids. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I. Used for the removal of DNA from protein samples.

DNase I是一种内切核酸酶，可作用于与嘧啶相邻的磷酸二酯键以产生具有末端5′-磷酸的聚核苷酸。当Mg 2+ 存在时，DNAse I可独立切割DNA的每条链且切割位点是随机的。当Mn 2+ 存在时，两条DNA链都几乎是在同样的位置被切割。 最适pH在7-8之间。二价阳离子如Mn 2+ , Ca 2+ , Co 2+ 以及Zn 2+ 都是酶的激活剂。 5 mM浓度的Ca 2+ 可稳定酶免收蛋白水解酶的消化。来自牛胰腺的DNAse I由四种色谱可区分的组分A，B，C和D组成，它们的摩尔比为4:1:1。仅有少量的D被发现。 2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS） 和肌动蛋白 都已知可抑制酶的活性。

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