DAB 底物

D755482-1T

1T

dark brown, /black precipitate; Visually evaluated

DAB 底物是过氧化物酶的有色底物，可用于多种应用：免疫组织细胞化学原位杂交蛋白质印迹斑点印迹DAB 底物已用于免疫荧光和蛋白质印迹。应用： 金属增强 DAB 底物在特殊配方中使用氯化钴和氯化镍，在辣根过氧化物酶（HRP）存在下产生深褐色/黑色沉淀。3, 3′二氨基联苯胺四盐酸盐（DAB）是一种应该安全使用的致癌物质。它是 HRP（辣根过氧化物酶）的主要敏感底物之一。DAB 呈棕色或琥珀色，由于过氧化氢和过氧化物酶的存在，变得不溶，并在抗原-抗体位点沉淀。 The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP). 3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase. Application: DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry In situ hybridization Western blot Dot blot DAB substrate has been used in immunofluorescence and western blot.