D-Glucose-1-13C can be used: • To predict the primary reaction mechanism in pyrolysis of glucose. • To study C−C bond cleavage mechanism in the conversion of labeled glucose into alkanediols using Ni−MgO−ZnO catalyst. • In tracer enrichment determination in blood plasma using high-resolution mass spectrometry. D-Glucose-1-13C can be used: • To predict the primary reaction mechanism in pyrolysis of glucose. • To study C−C bond cleavage mechanism in the conversion of labeled glucose into alkanediols using Ni−MgO−ZnO catalyst. • In tracer enrichment determination in blood plasma using high-resolution mass spectrometry.