Share to
Details
Safety
Related
Technical Documents
Articles
reviews
Consult
returns
Product details
名称
EZ-TA/Blunt 零背景 pTOPO II 克隆试剂盒
分子类型
试剂盒
英文名称
EZ-TA/Blunt Zero pTOPO II Cloning Kit
货号
E1372025-20T
包装规格
20T
级别
BioReagent
储存温度
-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
稳定性与储存
Store at -20℃ long term (12 months). Avoid freeze/thaw cycle.
产品介绍
本产品采用拓扑异构酶 I(Topoisomerase I)的连接原理,不同于使用 T4 DNA 连接酶的传统克隆方法,可在数分钟甚至数秒内高效连接 DNA 片段,可兼容 TA 克隆与平末端克隆。有效连接片段长度可达 10 kb。此外,本产品无自连、零背景,无需蓝白斑筛选,阳性克隆比例高,极少出现空载体,是简单、快速、零背景免筛选的 TOPO 通用型克隆试剂盒。载体上具有氨苄青霉素(Amp)和卡那青霉素(Kan)双抗性,有助于减少卫星菌落,便于后续挑取阳性克隆。试剂盒中提供 800 bp 的 DNA 片段(Control Insert)作为连接反应的阳性对照,同时提供 M13 F/R Primer Mix 通用型引物用于菌落 PCR 鉴定。 产品组分表 E1372025
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
Technical Documents
「No technical documents」
Articles
「No related articles yet」
user reviews
Loading...
Consultation

Warm reminder:

Because the manufacturer may temporarily adjust product specifications, prices, inventory, parameters and other information without notice, and there are differences in each user's consultation time and procurement needs, the platform reply content is only of reference value within a certain period. Sorry for the inconvenience caused to you, and thank you for your understanding and support! Please consult the online customer service for the latest details. Everything is subject to the real-time reply of the customer service.!

After Sales Services

Please check the quantity, appearance and product information in time when you receive the goods. In case of damage, wrong delivery, abnormal quality and other problems, please feel free to contact us.

Service Hotline:400-6226-992 ,We will handle it for you as soon as possible, thank you for your support and trust.!

Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:E1372025-20T
Product model:20T
level: BioReagent
Lead Time:30days
sold 405 Items
Ask Solution engineer
Specifications:

20T

Qty

Recommended for you