碱性磷酸酶 (AP) 的 ELISA 底物

E491262-100ml

100ml

medium

产品使用建议 培养温度：20-37 °C /68-100 °F 反应时间：在 20-37°C 中度（最多 30 分钟） 保质期：24 个月 有效成分及作用原理 在不同的缓冲溶液（pH = 9.5）中，根据需要添加不同的添加物，有效成分对硝基苯磷酸酯（pNPP）被溶解。 碱性磷酸酶将磷酸残基转移到受体上。在碱性条件下，由于形成的硝基苯酚会出现黄色。 主要优点 与新溶解的 pNPP 片剂相当的高吸光度产量 广泛的测量范围 低背景信号 长期储存期间空白漂移低（24 个月内 使用过的终止溶液终止反应后的高颜色稳定性 储存、运输和灌装信息 应避光储存在 2-8°C。在这些条件下，标签上印刷的保质期对未开封的瓶子有效。 自发衰减会增加背景。如果储存在室温下，衰变的速度会增加。因此，应避免在室温下储存和运输。然而，溶液的活性不受室温储存的影响。该解决方案在到期日之后仍然有效，但某些应用程序，尤其是包括视觉评估的应用程序，可能会因背景增加而受到阻碍。 从损坏的瓶子中污染或泄漏的底物溶液不应再使用，必须销毁。 使用带有一些冷藏袋的隔离容器进行运输。 应考虑以下装瓶说明： 在无尘和⿊暗的房间内工作。 尽可能保持溶液凉爽。 避免溶液与任何金属部件接触。 彻底清洁所有仪器和容器。 装瓶时戴上无粉手套。 立即关闭瓶子，以尽量减少光线和灰尘的影响。 使用由HDPE 或PP 制成的不透光的干净瓶子。 ELISA 中使用的⼀般说明 只有熟悉免疫学方法基础的合格实验室工作人员才能使用这些解决方案。 底物溶液可用于定性和定量 ELISA 程序。 使用 96 孔微量滴定板时，建议在孵育和洗涤后每孔加入 100 μL 纯底物。在底物温育之后，可以停止反应并且可以进行光度测量。使用更高的孵育温度 (37° C) 可能会缩短孵育时间。 停止2。使用其他市售停止解决方案不能安全地排除信号的进一步增加。添加终止溶液不会改变光谱的一般形状。 未停止和停止的溶液应在 405 nm 处测量，背景校正：应在 620 nm 处测量。 废弃物 废弃物的处置必须符合国家和地⽅的法律法规。包装的处置必须符合国家和地⽅的处置法规。 Product usage advice Incubation temperature: 20-37 °C / 68-100 °F Response time: Moderate (up to 30 minutes) at 20-37°C Shelf life: 24 months Active ingredients and principle of action In different buffer solutions (pH = 9.5), different additives were added as required, and the active ingredient p-nitrophenyl phosphate (pNPP) was dissolved. Alkaline phosphatase transfers phosphate residues to acceptors. Under alkaline conditions, a yellow color will appear due to the formation of nitrophenols. Main advantage High absorbance yield comparable to freshly dissolved pNPP tablets Wide measuring range low background signal Low blank drift during long-term storage ( High color stability after quenching the reaction with used stop solution Storage, Shipping and Filling Information Store at 2-8°C protected from light. Under these conditions, the expiration date printed on the label is valid for unopened bottles. Spontaneous decay increases the background. The rate of decay increases if stored at room temperature. Therefore, storage and transport at room temperature should be avoided. However, the activity of the solution was not affected by storage at room temperature. The solution remains valid beyond the due date, but some applications, especially those that include visual assessments, may be hindered by increased background. Contaminated or leaked substrate solutions from damaged bottles should not be reused and must be destroyed. Ship in an isolated container with some refrigerated bags. The following bottling instructions should be considered: Work in a dust-free and dark room. Keep the solution as cool as possible. Avoid contact of solution with any metal parts. Thoroughly clean all instruments and containers. Wear powder-free gloves when bottling. Close the bottle immediately to minimize the effects of light and dust. Use an opaque, clean bottle made of HDPE or PP. General instructions for use in ELISA Only qualified laboratory staff familiar with the basics of immunological methods should use these solutions. Substrate solutions can be used in qualitative and quantitative ELISA procedures. When using a 96-well microtiter plate, it is recommended to add 100 μL of pure substrate per well after incubation and washing. After substrate incubation, the reaction can be stopped and photometric measurements can be performed. Using a higher incubation temperature (37°C) may shorten the incubation time. stop 2. Further increases in signal cannot be safely ruled out using other commercially available stopping solutions. The addition of stop solution did not change the general shape of the spectrum. Unstopped and stopped solutions should be measured at 405 nm, background corrected: should be measured at 620 nm. Waste Disposal of waste must comply with national and local laws and regulations. Disposal of packaging must comply with national and local disposal regulations.