EC-17 (disodium salt) is a folate receptor alpha (FRα) targeting contrast agent with fluorescent properties in the visible light spectrum. The peak excitation and emission wavelengths of EC-17 are 470/520 nm. In Vitro EC-17 contains the fluorescein fluorochrome and has a spectral wavelength of 490-530?nm. EC-17 is synthesized by a folate (vitamin B9) and fluorescein isothiocyanate (FITC) conjugated through an ethylenediamine spacer to produce folate-FITC, with a molecular weight of 917?kDa. FITC is a derivative of fluorescein functionalized with an isothiocyanate reactive group. The folate-FITC conjugate forms a negatively charged fluorescent molecule that specifically targets cell-surface FRα and is subsequently internalized into the cytoplasm. The signal-to-background ratio (SBR) of EC-17 for HeLa cells range from 0.97 to 7.32 depending on the molarity and concentration of cancer cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. In Vivo The mean fluorescence signal from the animals injected with EC-17 to be 42,234 ± 12,234?au . Fluorescence microscopy for folate-FITC shows a strong signal in all malignant tumors with FR-α expression and no signal in FR-α–negative malignant or benign lesions. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid IC50& Target:Folate receptor alpha