EGF Receptor Substrate 2 (Phospho-Tyr5) is a biologically active peptide derived from an autophosphorylation site (Tyr 992 ) of epidermal growth factor receptor (EGFR). In Vitro DADEpYLIPQQG is a generalized PTPase substrate. Both CAV GST-VP2 and TLMV GST-ORF2 are shown to have PTPase activity using both ENDpYINASL and DADEpYLIPQQG. Steady state activities for the reaction of 5 μg of CAV GST-VP2 with ENDpYINASL and DADEpYLIPQQG are 100 and 208%, respectively, of those seen for 2 units of TC-PTP. In the context of DADEpYLIPQQG, the minimal sizes recognized by PTPα are either ADEpYLI or DADEpY-NH2. The k cat /K m value for the parent peptide DADEpYLIPQQG (EGFR 988-998 ) is 1090-fold higher than Tyr(P). MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid