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名称
增强型TMB显色液(ELISA HRP显色用)
货号
E743397-100ml
包装规格
100ml
浓度
过氧化物酶底物
产品介绍
阿拉丁该系列 单组份TMB ELISA 底物(单组份TMB显色液)具有低本底 、 稳定、 批间差小、 不同灵敏度可选的特点提供了优越的信号反应体系, 适用于 ELISA试剂盒 TMB BR为宽量程(Board Range)底物。 多用于定量检测或显色时间至20分钟的检测实验。 应用ELISA 。 本产品在使用前请平衡至反应温度。 显色步骤: 450nm 读值 : 1.洗板 ,在 HRP 酶标孵育后,用 PBST/TBST washing buffer(含表面活性剂)洗板3 次; 2.显色 ,加入 100μL 底物,在 20~37℃孵育一定时间,显色为蓝色; 3.终止 :加入 100μL TMB 终止液 450,显色变黄色; 4.读数 :建议在 30 分钟内读取波长 450 处的吸光值; 650nm 读值: 1.洗板 ,HRP 酶标孵育后,用 PBST/TBST washing buffer(含表面活性剂)洗板3 次; 2.显色 ,加入 100μL 底物,在 20~37℃孵育一定时间,显色为蓝色; 3.终止 :加入 100μL TMB 终止液 650,显色为蓝色; 4.读数 :建议在 30 分钟内读取波长 650 处的吸光值; 动力学 650nm 读值: 1.洗板 ,HRP 酶标孵育后,用 PBST/TBST washing buffer(含表面活性剂)洗板3 次; 2.显色 ,加入 100μL 底物,在 20~37℃孵育; 3. 读数 :按照既定的频率(如每隔 1 分钟)读取波长 650nm 处的吸光值; Precautions:Aladdin 's series of TMB ELISA substrates have the characteristics of low background, stability, small batch difference, and optional sensitivity. They provide superior signal reaction systems and are suitable for the development and production applications of ELISA kits.TMB Board Range One Component ELISA Substrate
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Research chemical 100ml

Research chemical 100ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 100ml.

item number:E743397-100ml
Product model:100ml
level: 100ml
Lead Time:30days
sold 966 Items
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