弹性蛋白酶 来源于人类白血球

E755335-1unit

1unit

lyophilized powder,≥50 units/mg protein (Bradford)

弹性蛋白酶（来源于人白细胞）已用于： 测定血清弹性蛋白酶活性纤连蛋白和唾液腺的蛋白水解消化中性粒细胞弹性蛋白酶(NE)活性测定E-钙粘蛋白的无细胞中性粒细胞弹性蛋白酶消化细胞划痕实验研究确定Nle3-血管紧张素(1-7)片段可加速皮肤模型的愈合弹性蛋白酶已用于消化纤连蛋白。将所得消化片段与人白细胞粗制匀浆的纤连蛋白消化片段进行比较，可用于研究干燥综合征患者唾液中纤连蛋白肽的存在。它还用作细胞裂解物的弹性蛋白酶活性测定的对照，用于研究全反式视黄酸对培养的原始细胞的促凝血和纤溶活性的影响，该原始细胞来自急性早幼粒细胞白血病患者。应用： 弹性蛋白酶是一种蛋白水解酶，属于肽基肽水解酶亚组成员。它是动脉的主要解剖组分。主要存在于各种鸟类和哺乳动物的胰腺和胰液中，还存在于人血清、粒细胞和红细胞中。 Elastase is a proteolytic enzyme. It is a member of the subgroup named, peptidyl peptide hydrolases. It is a major anatomic constituent of arteries. It is mainly found in the pancreas and pancreatic juice of various birds and mammals. It is also present in human serum, granulocytes and erythrocytes. Application: Elastase from human leukocytes has been used: to measure serum elastase activity in proteolytic digestion of fibronectin and salivary glands in neutrophil elastase (NE) activity assay cell-free NE digestion of E-cadherin scratch wound assay in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models Elastase has been used to digest fibronectin. The results were compared with fibronectin digestion by crude human leukocyte homogenate to examine the presence of fibronectin peptides in saliva of patients with Sjögren′s syndrome. It has also been used as a reference to determine the elastase activity in cell lysates. This study examined the effect of all-trans retinoic acid on procoagulant and fibrinolytic activities of cultured blast cells. These blast cells were from patients with acute promyelocytic leukemia.

弹性蛋白酶在蛋白水解过程的辅助下可从不溶性弹性蛋白纤维中释放可溶性多肽。 它在腺苷三磷酸(ATP)的辅助下可激活轴丝解体。 与胰腺弹性蛋白酶不同，白细胞酶优先切割缬氨酸的羧基侧，一定几率下也会在丙氨酸基团后进行切割。天然底物包括弹性蛋白、软骨蛋白聚糖、I、II、II和IV型胶原以及纤连蛋白。