弹性蛋白酶 来源于猪胰腺

E755348-10mg

10mg

Type I,≥4.0 units/mg protein

来自Sigma的酶已被用于开发弹性蛋白酶灌注的动物模型。该研究证明了烟草暴露是否可以降低AAA（腹主动脉瘤）发展所需的主动脉损伤阈值。它也被用于从人肺中分离出II型肺细胞。来自猪胰腺的弹性蛋白酶已用于研究弹性蛋白酶的仿生亲和配体的设计、合成和评价。来自猪胰腺的弹性蛋白酶也已用于研究胰蛋白水解酶（胰蛋白酶、胰凝乳蛋白酶和弹性蛋白酶）的纯化和部分表征。来自猪胰腺的弹性蛋白酶已被用于：诱发腹主动脉瘤（AAA）研究吲哚胺2-3双加氧酶1（IDO）对小鼠的影响消化主动脉以分离主动脉平滑肌细胞（SMC）作为蛋白水解消化的阳性对照 Elastase from porcine pancreas has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. Elastase from porcine pancreas has been used: to induce abdominal aortic aneurysm (AAA) to study the impact of indoleamine 2-3 dioxygenase 1 (IDO) in mice to digest aortas for aortic smooth muscle cells (SMC) isolation as a positive control of proteolytic digestion The enzyme from Sigma has been used in the development of elastase-perfused animal model . This study determined if tobacco exposure could lower the threshold of aortic injury necessary for AAA (abdominal aortic aneurysm) development. It has also been used during the isolation of type II pneumocytes from human lungs.

弹性蛋白酶是具有240个氨基酸残基的单一多肽链，其含有四个二硫键。分子量约为25.9kDa。该酶被合成为无活性的酶原形式，即原弹性蛋白酶，其随后通过胰蛋白酶在N-末端的有限蛋白水解而转化为活性形式。 它是一种具有广泛特异性的丝氨酸蛋白酶。它可切割在羧基侧具有小疏水性氨基酸（如Ile、Gly、Ala、Ser、Val和Leu）的蛋白质。该酶还可水解酰胺和酯，例如N-苯甲酰基-L-丙氨酸甲酯。 其最适pH为8.0-8.5。它不需要任何活化剂，但其活性可被二异丙基氟磷酸盐、苯基甲磺酰氟、α2-巨球蛋白、α1-抗胰蛋白酶、磺酰氟和对二硝基苯基二乙基磷酸盐、以及高盐浓度抑制。它广泛用于组织和细胞解离程序。弹性蛋白酶可有效分离II型肺细胞。弹性蛋白酶水解弹性蛋白（弹性纤维的特定蛋白质）并消化血红蛋白、酪蛋白和纤维蛋白。